Autor(es):
Schmidt, Elizabeth Moreira dos Santos [UNESP] ; Fachiolli, Daniele Floriano [UNESP] ; de Oliveira, Raphaela Moreira [UNESP] ; Almeida, Fabiana Alves [UNESP] ; Pariz, Cristiano Magalhães [UNESP] ; de Lima Meirelles, Paulo Roberto [UNESP] ; Costa, Ciniro [UNESP] ; Tvarijonaviciute, Asta ; Erel, Ozcan ; Neselioglu, Salim ; Ceron, Jose Joaquin ; Rubio, Camila Peres [UNESP]
Data: 2022
Identificador Persistente: http://hdl.handle.net/11449/229616
Origem: Oasisbr
Assunto(s): Antioxidant; Closantel; Haemonchus; Integrated crop-livestock; Oxidative stress; Ruminants
Descrição
Made available in DSpace on 2022-04-29T08:34:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-10-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
This work aimed to evaluate the thiol-disulphide homeostasis in serum of lambs naturally infected by gastrointestinal nematodes presenting different levels of parasite load indirectly indicated by faecal worm egg counts (EPG). Furthermore, the possible changes in the thiol-disulphide dynamic after different procedures to reduce the parasitic charge, such as the integrated crop-livestock system or anthelmintic treatment, were assessed. The results were compared with a panel of various oxidative stress and inflammatory biomarkers. The lambs were divided into three groups: animals highly infected (EPG higher than 5000) and packed cell volume (PCV) lower than 24% (G1); animals highly infected (EPG higher than 5000) and normal PCV (>24%) (G2); and animals presenting EPG lower than 5000 and normal PCV (>24%) (G3). The highly infected lambs (G1 and G2) showed lower total thiol (TT) and native thiol (SH) (p ≤ 0.01) than those from G3. After treatment, TT and SH increased significantly in all groups (p ≤ 0.01), and the disulphide (SS)/TT and SS/SH ratios decreased significantly (p < 0.01) in G1 and G2. These results show that the thiol-disulphide balance was impaired in lambs infected by gastrointestinal nematodes and that it could be potentially used as a biomarker to monitor this disease.
Department of Veterinary Clinical Sciences School of Veterinary Medicine and Animal Science Campus of Botucatu São Paulo State University (FMVZ-UNESP)
Faculty of Veterinary Medicine University Center of the Federal District-UDF
Department of Animal Nutrition and Breeding School of Veterinary Medicine and Animal Science Campus of Botucatu São Paulo State University (FMVZ-UNESP)
Department of Parasitology Biosciences Institute Campus of Botucatu São Paulo State University (FMVZ-UNESP)
Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU) Regional Campus of International Excellence ‘Campus Mare Nostrum’ University of Murcia, Espinardo
Department of Biochemistry Faculty of Medicine Ankara Yildirim Beyazit University Ankara City Hospital
Department of Veterinary Clinical Sciences School of Veterinary Medicine and Animal Science Campus of Botucatu São Paulo State University (FMVZ-UNESP)
Department of Animal Nutrition and Breeding School of Veterinary Medicine and Animal Science Campus of Botucatu São Paulo State University (FMVZ-UNESP)
Department of Parasitology Biosciences Institute Campus of Botucatu São Paulo State University (FMVZ-UNESP)
FAPESP: 2017/05055
