Autor(es): Salles, Bruno César Correa ; Leme, Krissia Caroline ; da Silva, Marcelo Aparecido ; da Rocha, Cláudia Quintino ; Tangerina, Marcelo M P [UNESP] ; Vilegas, Wagner [UNESP] ; Figueiredo, Sônia A ; Duarte, Stella Maris da Silveira ; Rodrigues, Maria Rita ; de Araújo Paula, Fernanda Borges
Data: 2022
Identificador Persistente: http://hdl.handle.net/11449/229693
Origem: Oasisbr
Assunto(s): Passiflora edulis; antiplatelet; diabetes; isoorientin; LC-MS quantification
Made available in DSpace on 2022-04-29T08:35:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-09-07
OBJECTIVES: This study was carried out to evaluate the effects of flavonoids present in leaves of Passiflora edulis fruit on complications induced by diabetes in rats. METHODS: The extract of P. edulis leaf was obtained by 70% ethanol maceration. From the dry extract, the fractions were obtained by consecutive liquid-liquid partition with hexane, ethyl acetate and n-butanol. The content of isoorientin of ethyl acetate and n-butanol fractions was determined by ultra-performance liquid chromatography coupled with electrospray and triple quadrupole ionization (TQD) analysis in tandem mass spectrometry (UPLC-ESI-Tq-MS). Only Fr-BuOH was used to treat diabetic or not Wistar rats. Biochemical parameters, platelet aggregation and production of reactive species were evaluated. KEY FINDINGS: The UPLC-ESI-Tq-MS analysis revealed the presence of several flavonoids, among which we identified five possible flavonoids c-heterosides (luteolin-7-O-pyranosyl-3-O-glucoside, apigenin-6-8-di-C-glycoside, apigenin-6-C-arabinoside-8-C-glycoside, isoorientin, isovitexin). The diabetic rats (treated intraperitoneally with alloxan, 150 mg/kg) treated with Fr-BuOH (20 mg/kg/day for 90 days) presented improvement in blood glucose, serum levels of fructosamine, lipid profile and urea. Furthermore, the Fr-BuOH reduced both platelet aggregation and the production of oxidant species in diabetic animals. CONCLUSIONS: These results suggested that flavonoid C-glycosides present in the Fr-BuOH may be beneficial for the diabetic state, preventing complications induced by diabetes.
Department of Clinical and Toxicological Analysis Faculty of Pharmaceutical Sciences Federal University of Alfenas
Department of Chemistry Federal University of Maranhão
Institute of Biosciences Coastal Campus of São Vicente Universidade Estadual Paulista
Department of Botany Institute of Biosciences University of São Paulo
Institute of Biosciences Coastal Campus of São Vicente Universidade Estadual Paulista
