Description
Made available in DSpace on 2013-09-27T14:53:33Z (GMT). No. of bitstreams: 1 WOS000289620100009.pdf: 394055 bytes, checksum: 9a23eb5b1d44e130bf137337fc894796 (MD5) Previous issue date: 2011-04-18
Made available in DSpace on 2013-09-30T18:01:03Z (GMT). No. of bitstreams: 1 WOS000289620100009.pdf: 394055 bytes, checksum: 9a23eb5b1d44e130bf137337fc894796 (MD5) Previous issue date: 2011-04-18
Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:17:54Z No. of bitstreams: 1 WOS000289620100009.pdf: 394055 bytes, checksum: 9a23eb5b1d44e130bf137337fc894796 (MD5)
Made available in DSpace on 2014-05-20T13:17:54Z (GMT). No. of bitstreams: 1 WOS000289620100009.pdf: 394055 bytes, checksum: 9a23eb5b1d44e130bf137337fc894796 (MD5) Previous issue date: 2011-04-18
Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
NIH (National Institutes of Health) - Fogarty
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Background: Corynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathogen, is the etiologic agent of the disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such as goats and sheep; it also causes infections in humans, though rarely. This species is distributed worldwide, but it has the most serious economic impact in Oceania, Africa and South America. Although C. pseudotuberculosis causes major health and productivity problems for livestock, little is known about the molecular basis of its pathogenicity.Methodology and Findings: We characterized two C. pseudotuberculosis genomes (Cp1002, isolated from goats; and CpC231, isolated from sheep). Analysis of the predicted genomes showed high similarity in genomic architecture, gene content and genetic order. When C. pseudotuberculosis was compared with other Corynebacterium species, it became evident that this pathogenic species has lost numerous genes, resulting in one of the smallest genomes in the genus. Other differences that could be part of the adaptation to pathogenicity include a lower GC content, of about 52%, and a reduced gene repertoire. The C. pseudotuberculosis genome also includes seven putative pathogenicity islands, which contain several classical virulence factors, including genes for fimbrial subunits, adhesion factors, iron uptake and secreted toxins. Additionally, all of the virulence factors in the islands have characteristics that indicate horizontal transfer.Conclusions: These particular genome characteristics of C. pseudotuberculosis, as well as its acquired virulence factors in pathogenicity islands, provide evidence of its lifestyle and of the pathogenicity pathways used by this pathogen in the infection process. All genomes cited in this study are available in the NCBI Genbank database (http://www.ncbi.nlm.nih.gov/genbank/) under accession numbers CP001809 and CP001829.
Fundação Oswaldo Cruz, Res Ctr Rene Rachou, Ctr Excellence Bioinformat, Natl Inst Sci & Technol, Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais (UFMG), Dept Gen Biol, Belo Horizonte, MG, Brazil
Fed Univ Para, Dept Genet, BR-66059 Belem, Para, Brazil
Univ Fed Sao Joao del Rei, Hlth Sci Ctr, Divinopilis, MG, Brazil
Univ Estadual Ceara, Dept Vet Med, Fortaleza, Ceara, Brazil
Univ Bielefeld, Dept Genet, CeBiTech, Bielefeld, Nordrhein Westf, Germany
Max Planck Inst Informat, Dept Comp Sci, Saarbrucken, Saarlan, Germany
Univ Fed Ouro Preto, Dept Pharmaceut Sci, Ouro Preto, MG, Brazil
Univ Fed Ouro Preto, Dept Phis, Ouro Preto, MG, Brazil
Univ Fed Triangulo Mineiro, Dept Biol Sci, Uberaba, MG, Brazil
Universidade Federal de Uberlândia (UFU), Dept Biochem & Genet, BR-38400 Uberlandia, MG, Brazil
Brazilian Agr Res Corp Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Sete Lagoas, MG, Brazil
Universidade Federal de Minas Gerais (UFMG), Dept Biochem & Immunol, Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais (UFMG), Dept Parasitol, Belo Horizonte, MG, Brazil
Univ Fed Ouro Preto, Dept Pharm, Ouro Preto, MG, Brazil
State Univ São Paulo, Dept Technol, Jaboticabal, SP, Brazil
Universidade Federal de Lavras (UFLA), Dept Chem, Lavras, MG, Brazil
Brazilian Agr Res Corp Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Campinas, SP, Brazil
Universidade Federal de Viçosa (UFV), Dept Plant Pathol, Vicosa, MG, Brazil
Universidade Federal da Bahia (UFBA), Dept Biointeract Sci, Salvador, BA, Brazil
CSIRO Livestock Ind, Clayton, Vic, Australia
State Univ São Paulo, Dept Technol, Jaboticabal, SP, Brazil
FAPEMIG: CBB-1181/0
FAPEMIG: REDE-186/08
NIH - Fogarty: TW007012
