Author(s):
Meyer, Wieland ; Castañeda, Alexandra ; Jackson, Stuart ; Huynh, Matthew ; Castañeda, Elizabeth ; Arechavala, Alicia ; Davel, Graciela ; Rodero, Laura ; Perrotta, Diego ; Lazera, Marcia ; Pereira-Igreja, Ricardo ; Wanke, Bodo ; Mendes-Giannini, Maria José Soares [UNESP] ; Melhem, Marcia S.C. ; Henning-Vainstein, Marlene ; Diaz, Maria Cristina ; Restrepo, Angela ; Huérfano, Sandra ; Samayoa, Blanca ; Logeman, Heidi ; Martirez, Rubén López ; Olivares, Laura Rocio Castañon ; Contreras-Peres, Cuadberto ; Tovar, José Francisco Valenzuela ; Bustamante, Beatriz ; Torres-Rodriquez, Joseph ; Morera, Yolanda ; Calvo, Belinda
Date: 2014
Persistent ID: http://hdl.handle.net/11449/67195
Origin: Oasisbr
Subject(s): controlled study; Cryptococcus neoformans; DNA fingerprinting; DNA polymorphism; fungal gene; fungus isolation; genetic analysis; geographic distribution; molecular typing; nonhuman; polymerase chain reaction; restriction fragment length polymorphism; serotype; ura5 gene; virus gene; Adult; Aged; Animals; Bacterial Typing Techniques; Central America; Cryptococcosis; DNA Fingerprinting; Epidemiology, Molecular; Female; Goats; Humans; Orotate Phosphoribosyltransferase; Parrots; Phylogeny; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; South America; Spain
Description
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A network was established to acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries. To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase chain reaction-fingerprinting and orotidine monophosphate pyrophosphorylase (URA5) gene restriction fragment length polymorphsm analysis with Hhal and Sau961 in a double digest. Both techniques grouped all isolates into eight previously established molecular types. The majority of the isolates, 68.2% (n=232), were VNI (var. grubii, serotype A), which accords with the fact that this variety causes most human cryptococcal infections worldwide. A smaller proportion, 5.6% (n=19), were VNII (var. grubii, serotype A); 4.1% (n=14), VNIII (AD hybrid), with 9 isolates having a polymorphism in the URA5 gene; 1.8% (n=6), VNIV (var. neoformans, serotype D); 3.5% (n=12), VGI; 6.2% (n=21), VGII; 9.1% (n=31), VGIII, and 1.5% (n=5) VGIV, with all four VG types containing var. gattii serotypes B and C isolates.
University of Sydney, Sydney, NSW
Instituto Nacional de Salud, Bogota
University of Western Sydney, Campbelltown, NSW
Molecular Mycology Laboratory CIDM Westmead Hospital, Darcy Road, Westmead, NSW 2145
Hosp. de Infecc. Francisco J. Muniz, Buenos Aires
Departamento Micologia Inst. Nac. Enferm. I. Dr. C.G.M., Buenos Aires
Laboratorio de Micologia Medica Hospital Evandro Chagas FundaVao Oswaldo Cruz, Rio de Janeiro
Faculdade de Ciencias Farmaceuticas Univ. Estadual Paulista (UNESP), Araraquara
Adolfo Lutz Inst. SeVao de Micologia, São Paulo
Centro de Biotechnologia (UFRGS), Porto Alegre
Prog. de Microbiologia y Micologia Universidad de Chile, Santiago
Corp. para Invest. Biologicas, Medellin
Instituto Nacional de Salud, Bogotá
Hospital San Juan de Dios, Guatemala City
Universidad de San Carlos, Guatemala City
Dept. de Microbiologia/Parasitologia Facultad de Medicina Univ. Nacional Autonoma de Mexico, México City
Inst. Nac. de Diagn./Ref. Epidemiol., Mexico City
Inst. de Med. Trop. A. Humboldt, Lima
Grup Recerca en Micologia Exp./Clin. Inst. Munic. d'Investigacio Medica Universitat Autonoma de Barcelona, Barcelona
Universidad del Zulia, Maracaibo
Faculdade de Ciencias Farmaceuticas Univ. Estadual Paulista (UNESP), Araraquara
