Author(s):
Silva, André Leandro ; Alexandrino Jr., Francisco ; Verissimo, Lourena Mafra ; Agnez-Lima, Lucymara Fassarella ; Egito, Lucila Carmem Monte ; Oliveira, Anselmo Gomes de [UNESP] ; do Egito, Eryvaldo Socrates Tabosa
Date: 2014
Persistent ID: http://hdl.handle.net/11449/73378
Origin: Oasisbr
Subject(s): Cationic lipid nanoemulsions; Gene therapy; Stearylamine; lipid; octadecylamine; plasmid DNA; complex formation; electrophoresis; incubation time; light scattering; low temperature; nanoemulsion; nonviral gene delivery system; particle size; temperature sensitivity; zeta potential
Description
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Cationic lipids have been used in the development of non-viral gene delivery systems as lipoplexes. Stearylamine, a cationic lipid that presents a primary amine group when in solution, is able to compact genetic material by electrostatic interactions. In dispersed systems such as nanoemulsions this lipid anchors on the oil/water interface confering a positive charge to them. The aim of this work was to evaluate factors that influence DNA compaction in cationic nanoemulsions containing stearylamine. The influence of the stearylamine incorporation phase (water or oil), time of complexation, and different incubation temperatures were studied. The complexation rate was assessed by electrophoresis migration on agarose gel 0.7%, and nanoemulsion and lipoplex characterization was done by Dynamic Light Scattering (DLS). The results demonstrate that the best DNA compaction process occurs after 120 min of complexation, at low temperature (4 ± 1 °C), and after incorporation of the cationic lipid into the aqueous phase. Although the zeta potential of lipoplexes was lower than the results found for basic nanoemulsions, the granulometry did not change. Moreover, it was demonstrated that lipoplexes are suitable vehicles for gene delivery. © 2012 by the authors; licensee MDPI, Basel, Switzerland.
Laboratório de Sistemas Dispersos (LASID) Universidade Federal do Rio Grande do Norte (UFRN), Natal/RN
Laboratório de Biologia Molecular e Genômica (LBMG) Departamento de Biologia Celular e Genética Centro de Biociências, Universidade Federal do Rio Grande do Norte (UFRN), Natal/RN
Laboratório de Analises Extratograficas Centro de Ciências Exatas e Tecnológicas Universidade Federal do Rio Grande do Norte (UFRN), Natal/RN
Departamento de Fármacos e Medicamentos Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista-Unesp, Rodovia Araraquara-Jaú, 14801-902, Araraquara, SP
Departamento de Fármacos e Medicamentos Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista-Unesp, Rodovia Araraquara-Jaú, 14801-902, Araraquara, SP
