Author(s): Paulan, Silvana de Cássia [UNESP] ; Gonzáles, Rutilia Marisela Hernándes ; Peralta, Laura Adalid ; Vicentini-Oliveira, Josy Campanhã [UNESP] ; Biondi, Germano Francisco [UNESP] ; Conde, Edda Sciuto ; Parkhouse, Robert Michael Evans ; Nunes, Caris Maroni [UNESP]
Date: 2014
Persistent ID: http://hdl.handle.net/11449/75020
Origin: Oasisbr
Subject(s): ELISA; Excretion-secretion; HP10 antigen assay; Sanitary inspection; Vesicular fluid
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Bovine cysticercosis, a cosmopolitan disease caused by Taenia saginata, leads to economic losses due to carcass devaluation at slaughter. Sanitary inspection at slaughterhouses, the routine diagnostic method in Brazil, lacks the necessary sensitivity to detect the mildly infected cattle that are typically encoutered in Brazil. In this study we have tested cattle sera from animals diagnosed as positive and negative by veterianry inspection for (1) anti-parasite antibodies using metacestodes antigens (T. solium vesicular fluid and T. saginata secretions) and (2) the HP10 secreted antigen of viable metacestodes. The cut-off values were calculated by ROC curve for intense and mild infections conditions, and by the classical method (X + 2DP for negative samples). The sensitivity and specificity of these diagnostic tests were different depending on the assumed cut-off value and, importantly, whether the infection was mild or intense. In spite of these observations, however, such ELISA assays for serum antibodies and parasite antigens constitute an important tool for epidemiological porposes, and in establishing priorities for the control of bovine cysticercosis.
Departamento de Apoio Produção e Saúde Animal Universidade Estadual Paulista 'Júlio de Mesquita Filho' - UNESP, Araçatuba, SP
Instituto de Investigaciones Biomédicas Universidad Nacional Autónoma de México, Ciudad de Mexico DF
Escola de Medicina Veterinária e Zootecnia Universidade Estadual Paulista 'Júlio de Mesquita Filho' - UNESP, Botucatu, SP
Instituto Gulbenkian de Ciências, Oeiras
Departamento de Apoio Produção e Saúde Animal Universidade Estadual Paulista 'Júlio de Mesquita Filho' - UNESP, Araçatuba, SP
Escola de Medicina Veterinária e Zootecnia Universidade Estadual Paulista 'Júlio de Mesquita Filho' - UNESP, Botucatu, SP
CNPq: 482681/2009-8
FAPESP: 09/12719-4
