Autor(es):
Oliveira, Edward José de ; Kanamura, HerminiaYohko ; Kioko Takei ; Hirata, Rosario Dominguez Crespo ; Valli, Luiz Carlos Pedrosa ; Nguyen, Nga Yen ; Rodrigues, Izabel de Carvalho ; Jesus, Amelia Ribeiro de ; Hirata, Mario Hiroyuki
Data: 2019
Origem: Oasisbr
Assunto(s): Schistosoma Mansoni; Diagnosis; Serology; Elisa; Synthetic Peptides; Sensitivity And Specificity; Antibodies; Infection; Sm31; Cysticercosis; Proteins; Chagas; Sera; Public, Environmental & Occupational Health; Tropical Medicine; Public, Environmental & Occupational Health; Tropical Medicine
Descrição
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A pool of five synthetic peptides was used as an antigenic base in an ELISA (ELISA-Pp) for laboratory diagnosis of Schistosoma mansoni. Serum samples were obtained from individuals with acute (n = 23) and chronic (n = 30) schistosomiasis, with other parasitoses (n = 39) or without parasitic infections (n = 100). ELISA-Pp was compared with other immunoenzymatic methods for detection of IgM (IgM-ELISA) or IgG (IgG-ELISA) as well as an immunofluorescence test for detection of IgM antibodies (IgM-IFT). The sensitivity and specificity of ELISA-Pp was 86.8% and 94.2% when tested on the schistosomiasis group and the non-schistosomiasis group, respectively. Comparison of ELISA-Pp with other serological methods resulted in K concordance indices varying from 0.59 to 0.75. Evaluation of anti-peptide IgG antibodies showed higher Levels in patients with acute compared with chronic schistosomiasis (P = 0.001). ELISA-Pp showed satisfactory sensitivity and high specificity and may constitute a potentially useful method for laboratory diagnosis of schistosomiasis mansoni. (c) 2007 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.
[de Oliveira, Edward Jose; Kanamura, HerminiaYohko; Kioko Takei; Hirata, Rosario Dominguez Crespo; Hirata, Mario Hiroyuki] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin, BR-05508900 Sao Paulo, SP, Brazil
[Kanamura, HerminiaYohko] Universidade de Taubaté (Unitau), Inst Basico Biociencias
[Valli, Luiz Carlos Pedrosa] Curso Farm, Fac Brasileira, Vitoria, ES, Brazil
[Nguyen, Nga Yen] US FDA, Ctr Biol Evaluat & Res, Bethesda, MD USA
[Rodrigues, Izabel de Carvalho] Inst Evandro Chagas, Secretaria Vigilancia Saude MS, Belem, Para, Brazil
[de Jesus, Amelia Ribeiro] Univ Fed Sergipe, Dept Med, Univ Hosp, Mol Biol Lab, Sergipe, Brazil
