Autor(es):
Braga, Adelaide ; Mesquita, D. P. ; Amaral, A. L. ; Ferreira, Eugénio C. ; Belo, Isabel
Data: 2015
Identificador Persistente: https://hdl.handle.net/1822/31632
Origem: RepositóriUM - Universidade do Minho
Assunto(s): Airlift bioreactor; Y. lipolytica; gamma-decalactone; Oxygen mass transfer rate; Cellular morphology; Image analysis; ?-Decalactone; γ-Decalactone; Science & Technology
Descrição
The production of γ-decalactone from castor oil in batch cultures of Yarrowia lipolytica W29 was compared in stirred tank (STR) and airlift bioreactors. Oxygen mass transfer from air to biphasic medium was characterized in both bioreactors trough correlations for kLa with power input and superficial gas velocity. Different conditions of oxygen transfer rate (OTR) were selected to perform biotransformations and for both bioreactors improvement of γ-decalactone productivity was obtained with OTR increase, with the highest value of 75 ± 10 mg L−1 h−1 being obtained in the STR for a kLa of 113 ± 5 h−1. However, a 2-fold increase in γ-decalactone concentration (around 3 g L−1) was achieved in the airlift compared to STR. Morphological characterization of the yeast cells by quantitative image analysis techniques showed that pneumatic agitation causes less impact in the cells morphology than mechanical agitation. A predominance of somewhat loose cells and quite irregular structures was observed in the STR.
The authors thank the Project 'Biolnd - Biotechnology and Bioengineering for improved Industrial and Agro-Food processes, REF. NORTE-07-0124-FEDER-000028' co-funded by the Programa Operacional Regional do Norte (ON.2 - O Novo Norte), QREN, FEDER, "BioEnv - Biotechnology and Bioengineering for a sustainable world", REF. NORTE-07-0124-FEDER-000048, co-funded by the Programa Operacional Regional do Norte (ON.2 - O Novo Norte), QREN, FEDER and Fundacao para a Ciencia e a Tecnologia (FCT) (SFRH/BD/63701/2009 PhD grant to Adelaide Braga and SFRH/BPD/82558/2011 post-doctoral grant to Daniela Mesquita) for the financial support provided and FCT Strategic Project PEst-OE/EQB/LA0023/2013.