Author(s):
Oliveira, Fernando E. ; Lima, C. ; Brás, S. ; França, Ângela ; Cerca, Nuno
Date: 2015
Persistent ID: http://hdl.handle.net/1822/38715
Origin: RepositóriUM - Universidade do Minho
Project/scholarship:
info:eu-repo/grantAgreement/FCT/3599-PPCDT/132966/PT;
info:eu-repo/grantAgreement/FCT/5876-PPCDTI/126270/PT
;
info:eu-repo/grantAgreement/FCT/COMPETE/126270/PT;
Subject(s): coagulase negative staphylococci; biofilm formation; flow cytometry; biofilm disruption assays; CLSM; Science & Technology
Description
Coagulase-negative staphylococci (CoNS) are common bacterial colonisers of the human skin. They are often involved in nosocomial infections due to biofilm formation in indwelling medical devices. While biofilm formation has been extensively studied in Staphylococcus epidermidis, little is known regarding other CoNS species. Here, biofilms from six different CoNS species were characterised in terms of biofilm composition and architecture. Interestingly, the ability to form a thick biofilm was not associated with any particular species, and high variability on biofilm accumulation was found within the same species. Cell viability assays also revealed different proportions of live and dead cells within biofilms formed by different species, although this parameter was particularly similar at the intra-species level. On the other hand, biofilm disruption assays demonstrated important inter- and intra-species differences regarding extracellular matrix composition. Lastly, confocal laser scanning microscopy (CLSM) experiments confirmed this variability, highlighting important differences and common features of CoNS biofilms. We hypothesised that the biofilm formation heterogeneity observed was rather associated with biofilm matrix composition than with cells themselves. Additionally, our results indicate that polysaccharides, DNA and proteins are fundamental pieces in the process of CoNS biofilm formation.
This work was co-funded by FCT Strategic Project PEst-OE/EQB/LA0023/2013, FCT project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462) and by QREN, FEDER, ON2 project NORTE-07-0124-FEDER-000027. NC is an Investigador FCT.
