Author(s):
Abreu, A. A. ; Tavares, F. ; Alves, M. M. ; Pereira, M. A.
Date: 2016
Persistent ID: https://hdl.handle.net/1822/42331
Origin: RepositóriUM - Universidade do Minho
Project/scholarship:
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F82000%2F2011/PT;
info:eu-repo/grantAgreement/FCT/5876/147337/PT;
info:eu-repo/grantAgreement/FCT/5876-PPCDTI/126270/PT
;
info:eu-repo/grantAgreement/FCT/COMPETE/126270/PT;
Subject(s): Thermotaga maritima; Caldicellulosiruptor saccharolyticus; Caldicellulosiruptor bescii; Co-cultures; Biohythane; Garden waste; Thermotoga maritima; Science & Technology
Description
Proof of principle of biohythane and potential energy production from garden waste (GW) is demonstrated in this study in a two-step process coupling dark fermentation and anaerobic digestion. The synergistic effect of using co-cultures of extreme thermophiles to intensify biohydrogen dark fermentation is demonstrated using xylose, cellobiose and GW. Co-culture of Caldicellulosiruptor saccharolyticus and Thermotoga maritima showed higher hydrogen production yields from xylose (2.7±0.1 mol mol -1 total sugar) and cellobiose (4.8±0.3 mol mol-1 total sugar) compared to individual cultures. Co-culture of extreme thermophiles C. saccharolyticus and Caldicellulosiruptor bescii increased synergistically the hydrogen production yield from GW (98.3±6.9 L kg-1 (VS)) compared to individual cultures and co-culture of T. maritima and C. saccharolyitcus. The biochemical methane potential of the fermentation end-products was 322±10 L kg-1 (CODt). Biohythane, a biogas enriched with 15% hydrogen could be obtained from GW, yielding a potential energy generation of 22.2 MJ kg-1 (VS).
The authors acknowledge the financial support of the Portuguese Science Foundation (FCT) and European Social Fund (ESF, POPH-QREN) through the grant given to A.A. Abreu (SFRH/ BPD/82000/2011). The authors thank the FCT Strategic Project of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145- FEDER-006684) and project RECI/BBB-EBI/0179/2012 (FCOMP-01- 0124-FEDER-027462).
