Autor(es): Vinayakumar, K. B. ; Silva, Maria Daniela ; Martins, Artur ; Mundy, Stephen ; González-Losada, Pedro ; Sillankorva, Sanna M.
Data: 2023
Identificador Persistente: https://hdl.handle.net/1822/85094
Origem: RepositóriUM - Universidade do Minho
Assunto(s): 3D printing; biofilms; Klebsiella pneumoniae; levofloxacin; microneedles; polyvinyl alcohol; Science & Technology
Additive manufacturing advancements contribute considerably to several fields, and its use in the medical field is gaining attention due to its easily customizable option (patient-specific), low cost, and fast turnout time in developing drug delivery and diagnostic tools. Here, wereport the fabrication of a microneedle (MN) platform using a stereolithography 3D printer, varying the 3D printing angle and aspect ratio (2:1, 3:1, 4:1). The optimal printing angle was30, resulting in needle tip and base diameters of 50 µm and 330 µm, and heights of 550/850/1180 µm. Polyvinyl alcohol (PVA) MNs produced with varying levofloxacin concentrations showed variability of 4% in tip and 3% base diameters and 15% in height compared to the 3D-printed MNs. Geometry B wasused to produce levofloxacin-loaded PVA MNs and tested against Klebsiella pneumoniae colony biofilms. Levofloxacin wasreleased gradually, as assessed by spectrofluorimetry. The MIC of levofloxacin against the K. pneumoniae clinical isolate was4 µg/mL, but this concentration wasinsufficient to cause any effect on K. pneumoniae biofilms. Only concentrations 32 µg/mL werestatistically different compared to the unloaded MNs. 3D printing is an attractive solution to produce molds for fabricating biopolymeric MNs for topical drug delivery.
S.S. acknowledges funding by FCT through the individual scientific employment program contract (2020.03171.CEECIND). The radius was corrected to diameter on June 12, 2023.
info:eu-repo/semantics/publishedVersion
