Author(s):
Braga, Cátia Sofia Neves ; Martins, Gilberto ; Duarte, Maria Salomé Lira ; Costa, João Carlos Sequeira ; Soares, O. S. G. P. ; Pereira, M. F. R. ; Pereira, I. C. ; Alves, M. M. ; Pereira, Luciana ; Salvador, Andreia Filipa Ferreira
Date: 2024
Persistent ID: https://hdl.handle.net/1822/93697
Origin: RepositóriUM - Universidade do Minho
Project/scholarship:
info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F132003%2F2017/PT;
info:eu-repo/grantAgreement/FCT/POR_NORTE/COVID%2FBD%2F152431%2F2022/PT;
info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F147271%2F2019/PT;
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT;
info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBTA-BTA%2F2249%2F2021/PT;
Subject(s): Methane; Materials; Anaerobic cultures
Description
Biomethane has a paramount role in the emergent energy transition occurring in Europe, and biotechnological strategies to accelerate biomethane production are needed. In this work, we investigated the effect of several materials (activated carbon (AC), magnetite and zeolite) on the activity of methanogenic cultures, including pure cultures and complex microbial communities. Anaerobic incubations were set up with and without materials and the effects on microbial activity were monitored by following methane production and investigating gene expression. Overall, the presence of materials had a positive effect with few exceptions. Nevertheless, we could not find a material with a broad-spectrum capacity to enhance methane production rate by all cultures tested. Metatranscriptomics analysis revealed no changes of typical syntrophic relationships in the presence of AC, as Syntrophomonadaceae microorganisms were the ones expressing the majority of the genes involved in butyrate degradation and hydrogen production, and hydrogenotrophic methanogens were highly abundant.
This work was supported by Portuguese Foundation for Science and Technology (FCT) through the grants SFRH/BD/132003/2017 and COVID/BD/152431/2022 attributed to Cátia Braga, and the grant SFRH/BD/147271/2019 attributed to João Sequeira. This research was also supported by FCT under the scope of the strategic funding of UIDB/04469/2020 unit and under the scope of the CM4Methane project (Ref: PTDC/BTA-BTA/2249/2021).
info:eu-repo/semantics/publishedVersion
