Author(s):
Trobajo-Sanmartín, Camino ; Ezpeleta, Guillermo ; Pais, Célia ; Eraso, Elena ; Quindós, Guillermo
Date: 2018
Persistent ID: https://hdl.handle.net/1822/65928
Origin: RepositóriUM - Universidade do Minho
Subject(s): Candida parapsilosis; Candidiasis, Invasive; DNA, Fungal; Genotyping Techniques; Humans; Multiplex Polymerase Chain Reaction; Mycological Typing Techniques; Sensitivity and Specificity; Microsatellite Repeats; Candida parapsilosis sensu stricto; Genotyping technique; Microsatellites repeats; Multiplex PCR; Specificity; Reproducibility of results
Description
Analysis of polymorphic microsatellite markers (STR) is a helpful genotyping technique to differentiate Candida parapsilosis sensu stricto isolates. The aim of this study is to develop and perform an initial validation of an alternative protocol for the reliable and accurate microsatellite genotyping of C. parapsilosis sensu stricto isolates using high-throughput multiplex PCR. To achieve this, the results obtained using the new protocol were compared to the ones obtained using a previously described reference method. To that end, diagnostic accuracy, informativeness and discrimination parameters were estimated.
