Author(s):
da Cunha, Aureliano Claret ; Gomes, Lorena Soares ; Godoy-Santos, Fernanda ; Faria-Oliveira, Fábio ; Teixeira, Janaína Aparecida ; Sampaio, Geraldo Magela Santos ; Trópia, Maria José Magalhães ; Castro, Ieso Miranda ; Lucas, Cândida ; Brandão, Rogelio Lopes
Date: 2019
Persistent ID: https://hdl.handle.net/1822/73415
Origin: RepositóriUM - Universidade do Minho
Subject(s): Aerobiosis; Alcoholic Beverages; Biofuels; Biomass; Bioreactors; Brazil; Candida; Chromatography, High Pressure Liquid; Cyanides; Ethanol; Fermentation; Food Technology; Glucose; Glycerol; Hydrogen-Ion Concentration; Industrial Microbiology; Kinetics; Protons; Saccharomyces cerevisiae; Saccharomycetales; Wickerhamomyces anomalus; Glycerol metabolism; Cachaca; Biotechnological applications; Glycerol transport; STL1
Description
Wickerhamomyces anomalus strain LBCM1105 was originally isolated from the wort of cachaça (the Brazilian fermented sugarcane juice-derived Brazilian spirit) and has been shown to grow exceptionally well at high amounts of glycerol. This paramount residue from the biodiesel industry is a promising cheap carbon source for yeast biotechnology. The assessment of the physiological traits underlying the W. anomalus glycerol consumption ability in opposition to Saccharomyces cerevisiae is presented. A new WaStl1 concentrative glycerol-H+ symporter with twice the affinity of S. cerevisiae was identified. As in this yeast, WaSTL1 is repressed by glucose and derepressed/induced by glycerol but much more highly expressed. Moreover, LBCM1105 aerobically growing on glycerol was found to produce ethanol, providing a redox escape to compensate the redox imbalance at the level of cyanide-resistant respiration (CRR) and glycerol 3P shuttle. This work is critical for understanding the utilization of glycerol by non-Saccharomyces yeasts being indispensable to consider their industrial application feeding on biodiesel residue.
