Autor(es):
Silva, Francisca Crislândia Oliveira ; Araújo, Maria Isabela Ferreira de ; Silva, Soraia P. ; Coelho, Elisabete ; Santos, Aline Teixeira dos ; Gonzalez, Abigail ; Salvador, Andreia Filipa Ferreira ; Cahú, Thiago Barbosa ; Vieira, Angélica Thomaz ; Souza, Marthyna Pessoa de ; Coimbra, Manuel A. ; Teixeira, J. A. ; Nobre, Clarisse ; Soares, Paulo Antônio Galindo ; Correia, Maria Tereza dos Santos
Data: 2025
Identificador Persistente: https://hdl.handle.net/1822/96022
Origem: RepositóriUM - Universidade do Minho
Assunto(s): Arabinogalactan; Chemical structure; Microbiota; Prebiotic
Descrição
This study aims to structurally characterize a polysaccharide extracted from Cenostigma nordestinum gum (AGCn) and evaluate its prebiotic potential in human gut microbiota. AGCn, obtained by dissolution in water and precipitation in 70 % ethanol, was primarily composed of carbohydrates (93.61 %), with a low protein content (1.70 %) and some phenolic compounds (9.16 % Gallic Acid Equivalents). Its molecular weight was 6.19 × 104 g/mol, with a dispersity of 1.33. The main monosaccharides identified were arabinose (38 mol%) and galactose (31 mol%). Methylation analysis and 2D NMR spectra showed that the gum exuded from C. nordestinum is a branched arabinogalactan composed of 6)--Galp-(1, 3,6)--Galp-(1, and 3,4,6)--Galp-(1 residues, containing chains of 3)--Araf-(1 and 5)--Araf-(1, with -Araf-(1 and -Rhap-(1 as terminal residues. The identification of 4)--GalpA-(1 showed also the presence of a pectic polysaccharide. AGCn was metabolized by Lactobacillus brevis, Lactobacillus plantarum, and Lactobacillus casei probiotic bacteria. AGCn in vitro fermentation increased the ratio of Bifidobacterium and Bacteroides in the microbiota, decreased the abundance of bacteria associated with intestinal disease, and produced short-chain fatty acids. Thus, AGCn is an arabinogalactan with prebiotic potential as a gut microbiota modulator, offering significant value for future food and biotechnological applications.
