Author(s):
da Costa, Paulo J. ; Menezes, Juliane ; Saramago, Margarida ; García-Moreno, Juan F. ; Santos, Hugo A. ; Gama-Carvalho, Margarida ; Arraiano, Cecília M. ; Viegas, Sandra C. ; Romão, Luísa
Date: 2020
Persistent ID: http://hdl.handle.net/10362/102671
Origin: Repositório Institucional da UNL
Project/scholarship:
info:eu-repo/grantAgreement/FCT/SFRH/BD/PT;
info:eu-repo/grantAgreement/FCT/SFRH/BD/PT;
info:eu-repo/grantAgreement/FCT/SFRH/BD/PT;
info:eu-repo/grantAgreement/FCT/SFRH/BPD/PT;
info:eu-repo/grantAgreement/FCT/UID/MULTI/PT;
info:eu-repo/grantAgreement/FCT/PTFC/BIM-MEC/PT;
Subject(s): DIS3L2; mRNA degradation; mRNA surveillance; NMD; NMD-targets; UPF1; General
Description
In this article, we present supportive data related to the research article “A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells” [1], where interpretation of the data presented here is available. Indeed, here we analyze the impact of the DIS3L2 exoribonuclease over nonsense-mediated mRNA decay (NMD)-targets. Specifically, we present data on: a) the expression of various reporter human β-globin mRNAs, monitored by Northern blot and RT-qPCR, before and after altering DIS3L2 levels in HeLa cells, and b) the gene expression levels of deregulated transcripts generated by re-analyzing publicly available data from UPF1-depleted HeLa cells that were further cross-referenced with a dataset of transcripts upregulated in DIS3L2-depleted cells. These analyses revealed that DIS3L2 regulates the levels of a subset of NMD-targets. These data can be valuable for researchers interested in the NMD mechanism.
