Autor(es):
Dias, Marta ; Pereira, Marta ; Marmelo, Isa ; Anacleto, Patrícia ; Pousão-Ferreira, Pedro ; Cunha, Sara C. ; Fernandes, José O. ; Petrarca, Mateus ; Marques, António ; Martins, Marta ; Maulvault, Ana Luísa
Data: 2024
Identificador Persistente: http://hdl.handle.net/10362/172615
Origem: Repositório Institucional da UNL
Projeto/bolsa:
info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FASP-PES%2F28708%2F2017/PT;
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04292%2F2020/PT;
info:eu-repo/grantAgreement/FCT/Concurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)/LA%2FP%2F0069%2F2020/PT;
info:eu-repo/grantAgreement/FCT/CEEC IND5ed/2022.07841.CEECIND%2FCP1724%2FCT0014/PT;
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT;
Assunto(s): Dietary exposure; Endocrine-disrupting chemicals; Fitness indicators; Gilthead seabream; Integrated biomarker response; Polybrominated diphenyl ethers; Environmental Engineering; Environmental Chemistry; Waste Management and Disposal; Pollution; SDG 14 - Life Below Water; SDG 15 - Life on Land
Descrição
Funding Information: This work was supported by FEDER (Programa Operacional Competitividade e Internacionalização - COMPETE 2020) from PIDDAC through FCT/MCTES project POCI-01–0145-FEDER-028708-PTDC/ASP-PES/28708/2017. All projects are funded by the Portuguese Foundation for Science and Technology (FCT)/Ministério da Ciência, Tecnologia e Ensino Superior (MCTES) . The authors are thankful to Sparos, Lda company for providing the fish feed, and to EPPO Aquaculture Research Station team (Olhão) for providing juvenile fish specimens. S.C.C. and I.M. acknowledge FCT for the Ph.D. Grant DFA/BD/4413/2020 , respectively. Publisher Copyright: © 2024 The Authors
Pentabromodiphenyl ether (BDE-99) and bisphenol A (BPA) are synthetic organic compounds present in several daily use products. Due to their physicochemical properties, they are ubiquitously present in aquatic ecosystems and considered highly persistent. Recent evidence has confirmed that both emerging compounds are toxic to humans and terrestrial mammals eliciting a wide range of detrimental effects at endocrine and immune levels. However, the ecotoxicological responses that they can trigger in vertebrate marine species have not yet been established. Hence, this study aimed to investigate the ecotoxicological responses of juvenile Sparus aurata upon chronic (28 days) dietary exposure to BDE-99 and BPA (alone and combined) following an integrated multi-biomarker approach that combined fitness indicators (Fulton's K and splenosomatic indexes) with endocrine [cortisol, 17β-estradiol (E2), 11-ketotestosterone (11-KT) concentrations] and immune (peroxidase and antiprotease activities) endpoints in fish plasma, and oxidative stress [superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) activities, and lipid peroxidation (LPO)] endpoints in the fish spleen. The mixture of BDE-99 and BPA yielded the highest IBR index value in both plasma and spleen biomarkers, therefore, suggesting that the effects of these compounds are more severe when they act together. Endocrine biomarkers were the most responsive in the three contaminated treatments. Fitness indicators were not affected by the individual nor the interactive effects of BDE-99 and BPA. These findings highlight the relevance of accounting for the interactive effects of emerging chemical contaminants and integrating responses associated with distinct biological pathways when investigating their impacts on marine life, as such a multi-biomarker approach provides a broader, more realistic and adequate perspective of challenges faced by fish in a contaminated environment.
