Autor(es):
Manjate, Filomena ; João, Eva D. ; Mwangi, Peter ; Chirinda, Percina ; Mogotsi, Milton ; Garrine, Marcelino ; Messa, Augusto ; Vubil, Delfino ; Nobela, Nélio ; Kotloff, Karen ; Nataro, James P. ; Nhampossa, Tacilta ; Acácio, Sozinho ; Weldegebriel, Goitom ; Tate, Jacqueline E. ; Parashar, Umesh ; Mwenda, Jason M. ; Alonso, Pedro L. ; Cunha, Celso ; Nyaga, Martin ; Mandomando, Inácio
Data: 2024
Identificador Persistente: http://hdl.handle.net/10362/178500
Origem: Repositório Institucional da UNL
Assunto(s): Children with diarrhoea; Children without diarrhoea; Manhiça district; Mozambique; Rotavirus group A; Whole genome sequencing; General; SDG 3 - Good Health and Well-being
Descrição
0.870\u2009\u2212\u200915 SC; The United States Agency for International Development (USAID), grant number AID-656-F-16-00002 and Fundo Nacional de Investiga\u00E7\u00E3o (FNI), Mo\u00E7ambique, grant number 245-INV funded the implementation of the surveillance of rotavirus and other enteropathogens in children less than 5 years of age in Manhi\u00E7a. The whole genome characterization undertaken in this study was supported by The Child Health and Mortality Prevention program (Surveillance), CHAMPS funded by the Bill & Melinda Gates Foundation under Grant OPP1126780; The Funda\u00E7\u00E3o para a Ci\u00EAncia e a Tecnologia for funds to GHTM-UID/04413/2020 and LA-REAL \u2013 LA/P/0117/2020, Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa, Portugal and the Next Generation Sequencing Unit, and the Division of Virology, Faculty of Health Sciences, University of the Free State, South Africa. The Calouste Gulbenkian Foundation financed F. M\u2019s PhD studies, under the grant number 234066. CISM receives core funding from the Mozambican government and the \u201CAgencia Espa\u00F1ola de Cooperacion Internacional para el Desarollo (AECID).\u201D Publisher Copyright: © The Author(s) 2024.
Post rotavirus vaccine introduction in Mozambique (September 2015), we documented a decline in rotavirus-associated diarrhoea and genotypes changes in our diarrhoeal surveillance spanning 2008–2021. This study aimed to perform whole-genome sequencing of rotavirus strains from 2009 to 2012 (pre-vaccine) and 2017–2018 (post-vaccine). Rotavirus strains previously detected by conventional PCR as G2P[4], G2P[6], G3P[4], G8P[4], G8P[6], and G9P[6] from children with moderate-to-severe and less-severe diarrhoea and without diarrhoea (healthy community controls) were sequenced using Illumina MiSeq® platform and analysed using bioinformatics tools. All these G and P-type combinations exhibited DS-1-like constellation in the rest of the genome segments as, I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis revealed that strains from children with and without diarrhoea clustered together with other Mozambican and global strains. Notably, the NSP4 gene of strains G3P[4] and G8P[4] in children with diarrhoea clustered with animal strains, such as bovine and caprine, with similarity identities ranging from 89.1 to 97.0% nucleotide and 89.5-97.0% amino acids. Our findings revealed genetic similarities among rotavirus strains from children with and without diarrhoea, as well as with animal strains, reinforcing the need of implementing studies with One Health approach in our setting, to elucidate the genetic diversity of this important pathogen.
