Author(s):
Marques, Isabel ; Fernandes, Isabel ; Paulo, Octávio S. ; Batista, Dora ; Lidon, Fernando C. ; Rodrigues, Ana P. ; Partelli, Fábio L. ; DaMatta, Fábio M. ; Ribeiro-Barros, Ana I. ; Ramalho, José C.
Date: 2024
Persistent ID: http://hdl.handle.net/10362/178920
Origin: Repositório Institucional da UNL
Project/scholarship:
info:eu-repo/grantAgreement/FCT/Concurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017/PTDC%2FASP-AGR%2F31257%2F2017/PT;
info:eu-repo/grantAgreement/FCT/3599-PPCDT/2022.01547.PTDC/PT;
info:eu-repo/grantAgreement/FCT/CEEC IND4ed/2021.01107.CEECIND%2FCP1689%2FCT0001/PT;
info:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Base/UIDB%2F00239%2F2020/PT;
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04035%2F2020/PT;
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0092%2F2020/PT;
Subject(s): climate changes; coffee; drought; functional analysis; heat; stress responses; Catalysis; Molecular Biology; Spectroscopy; Computer Science Applications; Physical and Theoretical Chemistry; Organic Chemistry; Inorganic Chemistry
Description
Funding Information: Fellowships from the Conselho Nacional de Desenvolvimento Cient\u00EDfico e Tecnol\u00F3gico, Brazil (CNPq) (FLP, FMD), and the Funda\u00E7\u00E3o de Amparo \u00E0 Pesquisa do Estado de Minas Gerais, Brazil (FAPEMIG, project CRA-RED-00053-16, APQ01512-18) (FMD), are also greatly acknowledged. Publisher Copyright: © 2024 by the authors.
Increasing exposure to unfavorable temperatures and water deficit imposes major constraints on most crops worldwide. Despite several studies regarding coffee responses to abiotic stresses, transcriptome modulation due to simultaneous stresses remains poorly understood. This study unravels transcriptomic responses under the combined action of drought and temperature in leaves from the two most traded species: Coffea canephora cv. Conilon Clone 153 (CL153) and C. arabica cv. Icatu. Substantial transcriptomic changes were found, especially in response to the combination of stresses that cannot be explained by an additive effect. A large number of genes were involved in stress responses, with photosynthesis and other physiologically related genes usually being negatively affected. In both genotypes, genes encoding for protective proteins, such as dehydrins and heat shock proteins, were positively regulated. Transcription factors (TFs), including MADS-box genes, were down-regulated, although responses were genotype-dependent. In contrast to Icatu, only a few drought- and heat-responsive DEGs were recorded in CL153, which also reacted more significantly in terms of the number of DEGs and enriched GO terms, suggesting a high ability to cope with stresses. This research provides novel insights into the molecular mechanisms underlying leaf Coffea responses to drought and heat, revealing their influence on gene expression.
