Preventing Clostridioides difficile infection by using an immobilized bile salt hydrolase to interfere with spore germination

• Author(s): Silva, Daniela
• Date: 2024
• Persistent ID: http://hdl.handle.net/10362/181944
• Origin: Repositório Institucional da UNL
• Subject(s): Bacillus subtilis; spore display; Lactobacillus salivarius; bile salt hydrolase; CotA
• Document Type: Master thesis
• Language: English
• Advisor(s): Oliveira Henriques, Adriano; Serrano, Mónica
• Contributor(s): RUN

Description

"The anaerobe Clostridioides difficile is a leading cause of healthcare-associated infections. C. difficile infection occurs after faecal-oral transmission of spores that germinate in the gut in response to certain bile salts. Commensal microbiota prevents spore germination by modulating the bile acid pool. Therefore, spore germination, toxin production and disease only occurs under dysbiosis, normally caused by antibiotic therapy. Lactobacillus salivarius is a commensal bacterium that produces a 76 kDa dimeric bile salt hydrolase (BSH). BSH catalyses the deconjugation of taurocholic acid in cholic acid and taurine and inhibits C. difficile spore germination and colonization in vivo.(...)"