Autor(es):
Lima, Katelene ; Malmir, Maryam ; Sabiha, Shabnam ; Pinto, Rui ; Silva, Isabel Moreira da ; Figueira, Maria Eduardo ; Rocha, João ; Duarte, Maria Paula ; Silva, Olga
Data: 2025
Identificador Persistente: http://hdl.handle.net/10362/186638
Origem: Repositório Institucional da UNL
Projeto/bolsa:
info:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Programático/UIDP%2F04138%2F2020/PT;
info:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Base/UIDB%2F04077%2F2020/PT;
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04077%2F2020/PT;
Assunto(s): Antiglycation; Diabetes; DPP4; Herbal medicine; Periploca chevalieri; α-amylase; α-glucosidase; Molecular Medicine; Pharmaceutical Science; Drug Discovery; SDG 3 - Good Health and Well-being
Descrição
Funding Information: This research was funded by the Foundation for Science and Technology (FCT, Portugal) through national funds to iMed.ULisboa (UIDP/04138/2020) and to the Mechanical Engineering and Resource Sustainability Center—MEtRICs unit, which is funded by national funds from FCT/MCTES (UIDB/04077/2020 and UIDP/04077/2020). Publisher Copyright: © 2025 by the authors.
Background/Objectives: Periploca chevalieri Browicz (Apocynaceae), an endemic species of the Cabo Verde archipelago, is commonly used in traditional medicine for the treatment of diabetes. The aim of this study was to characterize the chemical profiles of the aqueous and hydroethanolic (70%) extracts of the P. chevalieri dried aerial parts (PcAE and PcEE) and evaluate their potential to modulate postprandial glycemia and inhibit key carbohydrate-hydrolyzing enzymes. Methods: The chemical characterization was performed by LC/UV-DAD-ESI/MS/MS. An in vivo evaluation of postprandial glycemia modulation was conducted on healthy CD1 mice submitted to an oral sucrose tolerance test. In vitro enzymatic inhibition was performed for the α-amylase, α-glucosidase, and DPP4 enzymes. Additionally, antioxidant and antiglycation activities were also assessed. Results: Phenolic acid derivatives, flavanols, proanthocyanidins, and flavonols were the major classes of secondary metabolites identified. PcEE at 170 mg/kg of body weight significantly (p < 0.05) reduced the postprandial glycemia peak in CD1 mice submitted to sucrose overload. Regarding the enzymatic inhibition, both extracts showed concentration-dependent inhibitory potential against the α-amylase, α-glucosidase, and DPP4 enzymes. Both extracts inhibited α-glucosidase more effectively than acarbose. Conclusions: The obtained results supports the traditional use of P. chevalieri and suggest the potential for further pharmacological investigation.
