Autor(es): Braz, Luis ; Grenha, Ana ; Corvo, Marta C. ; Lourenço, João P. ; Ferreira, Domingos ; Sarmento, Bruno ; Costa, Ana M. Rosa da
Data: 2018
Identificador Persistente: http://hdl.handle.net/10400.1/10376
Origem: Sapientia - Universidade do Algarve
Assunto(s): Carboxylated locust bean gum; Polyelectrolyte complexation; Polymeric nanoparticles; Sulfated locust bean gum; Tetraalkylammonium locust bean gum
The development of LBG-based nanoparticles intending an application in oral immunization is presented. Nanoparticle production occurred by mild polyelectrolyte complexation, requiring the chemical modification of LBG. Three LBG derivatives were synthesized, namely a positively charged ammonium derivative (LBGA) and negatively charged sulfate (LBGS) and carboxylate (LBGC) derivatives. These were characterized by Fourier-transform infrared spectroscopy, elemental analysis, nuclear magnetic resonance spectroscopy, gel permeation chromatography, and x-ray diffraction. As a pharmaceutical application was aimed, a toxicological analysis of the derivatives was performed by both MTT test and LDH release assay. Several nanoparticle formulations were produced using LBGA or chitosan (CS) as positively charged polymers, and LBGC or LBGS as negatively charged counterparts, producing nanoparticles with adequate properties regarding an application in oral immunization.
