Autor(es): Menezes,Caroline Anunciação ; Gonçalves,Danielle Silva ; Amianti,Jackeline ; Fernandes,Irene ; Taddei,Carla Romano ; Koga,Paula Célia Mariko ; Trabulsi,Luiz Rachid ; Martinez,Marina Baquerizo ; Piazza,Roxane Maria Fontes
Data: 2003
Origem: Oasisbr
Assunto(s): detection; polyclonal antibody; monoclonal antibody; thermo-labile toxin; E. coli
A capture enzyme-linked immunosorbent assay (ELISA), which detects LT-I toxin produced by enterotoxigenic Escherichia coli strains, has beendeveloped. This capture assay was performed using the IgG enriched fraction of anti-LT-I antiserum and IgG2b anti-LT-I monoclonal antibody and allowed a clear distinction between E. coli LT-I - producing and non-producing strains. The estimated accuracy of the assay is 78% for sensitivity, 94% for specificity and 92% for efficiency. Thus, the capture immunoassayis a sensitive tool for detection of E. coli, which produces heat-labile enterotoxin, and is suitable for use in clinical laboratories and epidemiological surveys in developing world.
