Author(s): Bernardes, Nuno ; Ribeiro, Ana Sofia ; Abreu, Sofia ; Vieira, André F. ; Carreto, Laura ; Santos, Manuel ; Seruca, Raquel ; Paredes, Joana ; Fialho, Arsenio M.
Date: 2014
Persistent ID: http://hdl.handle.net/10773/18781
Origin: RIA - Repositório Institucional da Universidade de Aveiro
Subject(s): Azurin; Breast cancer; Integrin; P-cadherin; cDNA microarrays
Azurin is a bacterial protein from Pseudomonas aeruginosa which exerts an inhibitory activity in cancer cells. In P-cadherin-overexpressing models, a bad prognosis marker in breast cancer increasing invasion and other malignant features, azurin decreases the invasion of cancer cells. We performed a microarray analysis to compare the expression profile of azurin treated cells with different P-cadherin expression levels. Azurin up-regulated apoptosis mediated by p53 protein, endocytosis and vesicle-mediated transport. In the contrary, in invasive MCF-7/AZ.Pcad cells, azurin decreased the expression of genes associated with cell surface receptors and signal transduction, as well as biological adhesion. Further, azurin decreased adhesion of cells to proteins from the extracellular matrix (ECM) and altered protein expression of integrins α6, β4 and β1 and interfered with the ability of these cells to form mammospheres. Altogether, our results further enlighten the anti-cancer effects mediated by azurin in P-cadherin overexpression breast cancer models.
