12 documents found, page 1 of 2
Electrospun nanofibrous meshes cultured with Wharton’s Jelly Stem Cell: an alte...
Silva, Marta Luísa Sousa Dias Alves; Martins, Albino; Costa-Pinto, A. R.; Monteiro, Nelson; Faria, Susana; Reis, R. L.; Neves, N. M.Many efforts are being directed worldwide to the treatment of OA-focal lesions. The majority of those efforts comprise either the refinement of surgical techniques or combinations of biomaterials with various autologous cells. Herein, we tested electrospun polycaprolactone (PCL) nanofibrous meshes for cartilage tissue engineering. For that, articular chondrocytes (hACs) isolated from human osteoarthritic joints...
Is wintertime air quality in Évora deteriorating as a consequence of the econom...
Monteiro, Nelson; Malico, Isabel; Pereira, Sérgio; Costa, Maria JoãoThe serious economical crisis that affects some European countries, among these Portugal, may be leading to the replacement of the conventional household heating methods by less expensive inefficient wood burning. This has already been demonstrated in Greece and may constitute a severe air quality problem in some regions in the cold periods of the year, due to the release of dangerous substances into the atmosp...
On the use of dexamethasone-loaded liposomes to induce the osteogenic different...
Monteiro, Nelson; Martins, Albino; Ribeiro, Diana Margarida da Costa; Faria, Susana; Fonseca, Nuno A.; Moreira, João N.; Reis, R. L.; Neves, N. M.Article first published online: 7 Oct. 2013; Stem cells have received considerable attention by the scientific community because of their potential for tissue engineering and regenerative medicine. The most frequently used method to promote their differentiation is supplementation of the in vitro culture medium with growth/differentiation factors (GDFs). The limitations of that strategy caused by the short half...
Nanoparticle-based bioactive agent release systems for bone and cartilage tissu...
Monteiro, Nelson; Martins, Albino; Reis, R. L.; Neves, N. M.The inability to deliver bioactive agents locally in a transient but sustained manner is one of the challenges on the development of bio-functionalized scaffolds for tissue engineering (TE) and regenerative medicine. The mode of release is especially relevant when the bioactive agent is a growth factor (GF), because the dose and the spatiotemporal release of such agents at the site of injury are crucial to achi...
Antibacterial activity of chitosan nanofiber meshes with liposomes immobilized ...
Monteiro, Nelson; Martins, Margarida; Martins, Albino; Fonseca, Nuno A.; Moreira, João N.; Reis, R. L.; Neves, N. M.Chitsan (Ch) nanofiber mesh (NFM) is a material with natural characteristics favoring its use in human wound dressing. The present work proposes a gentamicin-loaded liposome immobilized at the surface of Ch NFMs to promote its antibacterial activity. To achieve this purpose, Ch NFMs were functionalized with thiol groups, and gentamicin-loaded liposomes were covalently immobilized by the reaction of the SH group...
Immobilization of bioactive factor-loaded liposomes on the surface of electrosp...
Monteiro, Nelson; Martins, Albino; Pires, R. A.; Faria, Susana; Fonseca, Nuno A.; Moreira, João N.; Reis, R. L.; Neves, N. M.Electrospun nanofiber meshes (NFM), due to their morphology and fibrous structure, are extensively proposed as biomedical devices, for tissue engineering on scaffolds and also as drug delivery systems. Liposomes are nanoparticles prepared from a biologically derived material (phospholipid), which are already in clinical use as a drug release device. Liposomes may be combined with biomaterial scaffolds to promot...
Liposomes in tissue engineering and regenerative medicine
Monteiro, Nelson; Martins, Albino; Reis, R. L.; Neves, N. M.Liposomes are vesicular structures made of lipids that are formed in aqueous solutions. Structurally, they resemble the lipid membrane of living cells. Therefore, they have been widely investigated, since the 1960s, as models to study the cell membrane, and as carriers for protection and/or delivery of bioactive agents. They have been used in different areas of research including vaccines, imaging, applications...
Advanced antibacterial wound dressing produced with natural-origin materials
Monteiro, Nelson; Martins, Margarida Isabel Barros Coelho; Martins, Albino; Fonseca, Nuno A.; Moreira, João N.; Reis, R. L.; Neves, N. M.Portuguese Foundation for Science and Technology for the OsteoGraphy (PTDC/EME- MFE/2008) and MaxBone (PTDC/SAU -ENB/115179/2009) project. This work was partly supported by “RL1 - ABMR - NORTE-01-0124- FEDER-000016” cofinanced by North Portugal Regional Operational Programme (ON.2 – O Novo Norte), under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF) proj...
Instructive nanofibrous scaffold comprising runt-related transcription factor 2...
Monteiro, Nelson; Ribeiro, Diana Margarida da Costa; Martins, Albino; Faria, Susana; Fonseca, Nuno A.; Moreira, João N.; Reis, R. L.; Neves, N. M."Epub 2014 Jul. 30"; Inducer molecules capable of regulating mesenchymal stem cells (MSCs) differentiation into specific lineages have proven effective in basic science and in preclinical studies. Runt-related transcription factor 2 (RUNX2) is considered to be the central gene involved in the osteoblast phenotype induction, which may be advantageous for inducing bone tissue regeneration. This work envisions the...
Development of advanced release systems for stem cell based therapies
Monteiro, NelsonTese de doutoramento do Programa Doutoral em Bioengenharia; The increasing incidence of bone, cartilage, tendon and ligament injuries is stimulating the development of less invasive surgical procedures and of treatment modalities that overcome the limitations of conventional therapies, while enhancing the functional recovery. Tissue Engineering and Regenerative Medicine (TERM) strategies are promising alternati...