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In this study a pure tone audiogram was developed under the Matlab® mathematical software. Audiogram measurements were performed to 35 subjects belonging to the female and male and aged between 10 and 88 years old. Some of the subjects with more advanced age had hearing problems over the course of age, however, none of them was carrying any type of hearing aid. The threshold of the Sound Pressure Level (SPL) was recorded under 12 pure tones between 125 Hz and 15 kHz. The developed pure-tone audiogram confirmed its ability to produce auditory brainstem responses (ABRs). Statistical analysis of the SPL threshold shows no differences between genders and confirms the correlation between age and loss of sensitivity, more accentuated for higher frequency tones. A strong loss of sensitivity was observed after the decade of 60 years old.