Detalhes do Documento

Cytogenetic study of Anopheles albitarsis (Diptera: Culicidae) by C-banding and in situ hybridization

Autor(es): Rafael, Míriam Silva ; Santos, I. P. ; Tadei, Wanderli Pedro ; Carvalho, Kenia A. ; Recco-Pimente, S. M. ; Sallum, Maria Anice Mubeb ; Forattini, Oswaldo Paulo

Data: 2020

Origem: Oasisbr

Assunto(s): Anopheles; Anopheles Albitarsis; Brasil; C Banding; Centromere; Chromosome Nor; Cytogenetics; Female; In Situ Hybridization, Fluorescence; Heterochromatin; Malaria; Nonhuman; X Chromosome; Y Chromosome; Animal; Anopheles; Brasil; Chromosome Banding; Chromosomes; In Situ Hybridization, Fluorescence; Karyotyping; Metaphase; X Chromosome; Y Chromosome; Anopheles Albitarsis; Culicidae; Diptera; Anopheles; Anopheles; Anopheles Albitarsis; Anopheles Albitarsis; Brasil; Brasil; C Banding; C Banding; Centromere; Centromere; Chromosome Nor; Chromosome Nor; Cytogenetics; Cytogenetics; Female; Female; In Situ Hybridization, Fluorescence; In Situ Hybridization, Fluorescence; Heterochromatin; Heterochromatin; Malaria; Malaria; Nonhuman; Nonhuman; X Chromosome; X Chromosome; Y Chromosome; Y Chromosome; Animal; Animal; Anopheles; Anopheles; Brasil; Brasil; Chromosome Banding; Chromosome Banding; Chromosomes; Chromosomes; In Situ Hybridization, Fluorescence; In Situ Hybridization, Fluorescence; Karyotyping; Karyotyping; Metaphase; Metaphase; X Chromosome; X Chromosome; Y Chromosome; Y Chromosome; Anopheles Albitarsis; Anopheles Albitarsis; Culicidae; Culicidae; Diptera; Diptera


Descrição

The C-banding pattern and the size and location of the nucleolar organizer regions (NORs) are described for the first time in Brazilian populations of Anopheles (Nyssorhynchus) albitarsis sensu lato. C-banding revealed variation in the size of the centromeric heterochromatic blocks in autosomal chromosomes and in the acrocentric (X) and puntiform (Y) sex chromosomes. Fluorescence in situ hybridization showed that the NORs were located in the pericentromeric region of the sex (XX/XY) chromosomes and that this coincided with the number and location of centromeric constitutive heterochromatin blocks previously revealed by C-banding. The NORs varied in size among the homologues of the three populations. These findings of the populations studied support the hypothesis that the stability of NORs in the A. albitarsis complex is characterized by the presence of clustered and conserved sites in a unique pair of chromosomes.

Tipo de Documento Artigo científico
Idioma Inglês
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