Author(s):
Kreling, Paula Fernanda [UNESP] ; Aida, Kelly Limi [UNESP] ; Massunari, Loiane [UNESP] ; Caiaffa, Karina Sampaio [UNESP] ; Percinoto, Célio [UNESP] ; Bedran, Telma Blanca Lombardo [UNESP] ; Spolidorio, Denise Madalena Palomari [UNESP] ; Abuna, Gabriel Flores ; Cilli, Eduardo Maffud [UNESP] ; Duque, Cristiane [UNESP]
Date: 2018
Persistent ID: http://hdl.handle.net/11449/173532
Origin: Oasisbr
Subject(s): Biofilms; Cell culture; Dental caries; Peptides; Biofilms; Biofilms; Cell culture; Cell culture; Dental caries; Dental caries; Peptides; Peptides
Description
Made available in DSpace on 2018-12-11T17:06:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-08-18
This study evaluated the cytotoxicity and effect of fragments derived from three oral cationic peptides (CP): LL-37, D6-17 and D1-23 against cariogenic bacteria under planktonic and biofilm conditions. For cytotoxicity analysis, two epithelial cell lines were used. The minimum inhibitory concentration and the minimal bactericidal concentration were determined for the CP fragments and the control (chlorhexidine-CHX) against cariogenic bacteria. The fractional inhibitory concentration was obtained for the combinations of CP fragments on Streptococcus mutans. Biofilm assays were conducted with the best antimicrobial CP fragment against S. mutans. The results indicated that D6-17 was not cytotoxic. D1-23, LL-37 and CHX were not cytotoxic in low concentrations. D1-23 presented the best bactericidal activity against S. mutans, S. mitis and S. salivarius. Combinations of CP fragments did not show a synergic effect. D1-23 presented a higher activity against S. mutans biofilm than CHX. It was concluded that D1-23 showed a substantial effect against cariogenic bacteria and low cytotoxicity.
Department of Pediatric Dentistry and Public Health Araçatuba Dental School UNESP - Universidade Estadual Paulista
Department of Restorative Dentistry (Endodontics) Araçatuba Dental School UNESP - Universidade Estadual Paulista
Department of Physiology and Pathology Araraquara Dental School UNESP - Universidade Estadual Paulista
Department of Dental Materials Piracicaba Dental School UNICAMP - Universidade de Campinas
Department of Biochemistry and Chemical Technology Institute of Chemistry UNESP - Universidade Estadual Paulista
Department of Pediatric Dentistry and Public Health Araçatuba Dental School UNESP - Universidade Estadual Paulista
Department of Restorative Dentistry (Endodontics) Araçatuba Dental School UNESP - Universidade Estadual Paulista
Department of Physiology and Pathology Araraquara Dental School UNESP - Universidade Estadual Paulista
Department of Biochemistry and Chemical Technology Institute of Chemistry UNESP - Universidade Estadual Paulista
