Autor(es):
Costa, Victor [UNESP] ; El-Achkar, Vivian Narana [UNESP] ; de Barros, Patrícia Pimentel [UNESP] ; León, Jorge Esquiche ; Ribeiro-Silva, Alfredo ; Carlos, Román ; Pignatari, Shirley Shizue Nagata ; Ferreira, Silvaneide ; Mello, Barbara Pereira ; Sichero, Laura ; Villa, Luisa Lina ; Kaminagakura, Estela [UNESP]
Data: 2020
Identificador Persistente: http://hdl.handle.net/11449/199858
Origem: Oasisbr
Assunto(s): CD21; Epstein-Barr virus; human papilloma virus; severity; Squamous papillomatosis; CD21; CD21; Epstein-Barr virus; Epstein-Barr virus; human papilloma virus; human papilloma virus; severity; severity; Squamous papillomatosis; Squamous papillomatosis
Descrição
Made available in DSpace on 2020-12-12T01:51:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01
Objective: The objective was to investigate the prevalence of the Epstein-Barr virus (EBV) and its association with human papilloma virus (HPV) detection, clinicopathological features, and the severity of recurrent respiratory papillomatosis (RRP). Methods: Cases of juvenile recurrent respiratory papillomatosis (JRRP) (n = 36) and adult recurrent respiratory papillomatosis (ARRP) (n = 44) were collected retrospectively and subdivided into low- and high-risk severity groups based on the Derkay score. We performed HPV detection and genotyping using a reverse hybridization protocol and investigated the presence of EBV by polymerase chain reaction (PCR) and in situ hybridization. CD21 levels were accessed by immunohistochemistry. Results: All samples were HPV-positive, including 49 cases of HPV 6, 26 cases of HPV 11, four cases of HPV 6 and 11 coinfections, and one case of HPV 16. EBV-DNA was detected in nine samples by PCR, although none of the cases were positive by means of in situ hybridization. CD21 immunoexpression was not statistically associated with any of the variables analyzed. HPV 6 detection was significantly higher in ARRP cases (P = 0.03), whereas HPV 11 was more prevalent in JRRP cases (P = 0.02) and was even more prevalent in JRRP cases of greater severity (Derkay laryngoscopic scale ≥20) (P = 0.04). Conclusion: The presence of EBV does not seem to play an important role in the progression/severity of RRP. Level of Evidence: 4 Laryngoscope, 2019.
Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (Unesp), São José dos Campos
Department of Stomatology Collective Health and Legal Dentistry Ribeirão Preto School of Dentistry
Department of Pathology and Forensic Medicine Ribeirão Preto Medical School University of São Paulo Ribeirão Preto
Department of Otolaryngology and Head and Neck Surgery Federal University of Sao Paulo
Center for Translational Investigation in Oncology Instituto do Cancer do Estado de Sao Paulo Hospital das Clinicas da Faculdade de Medicina da Universidade de São Paulo
Department of Radiology and Oncology Faculdade de Medicina Universidade de São Paulo
Centro Clínico de Cabeza y Cuello
Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (Unesp), São José dos Campos
