Author(s):
de Lima, Laura C. [UNESP] ; Ramos, Matheus A. S. [UNESP] ; de Toledo, Luciani G. [UNESP] ; Rodero, Camila F. [UNESP] ; Hilário, Felipe [UNESP] ; Dos Santos, Lourdes C. [UNESP] ; Chorilli, Marlus [UNESP] ; Bauab, Taís M. [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/200506
Origin: Oasisbr
Subject(s): Antifungal; Candida parapsilosis; Candida sp; Lipid nanoemulsion; Syngonanthus nitens; Vulvovaginal candidiasis; Antifungal; Antifungal; Candida parapsilosis; Candida parapsilosis; Candida sp; Candida sp; Lipid nanoemulsion; Lipid nanoemulsion; Syngonanthus nitens; Syngonanthus nitens; Vulvovaginal candidiasis; Vulvovaginal candidiasis
Description
Made available in DSpace on 2020-12-12T02:08:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Background: Vaginal infections caused by non-albicans species have become common in women of all age groups. The resistance of species such as Candida parapsilosis to the various antifungal agents is a risk factor attributed to these types of infections, which instigates the search for new sources of active compounds in vulvovaginal candidiasis (VCC) therapy. Objective: This study evaluated the antifungal activity of Syngonanthus nitens Bong. (Ruhland) derivatives and employed a lipid nanoemulsion as a delivery system. Methods: In this study, a lipid nanoemulsion was employed as a delivery system composed of Cholesterol (10%), soybean phosphatidylcholine: Brij 58 (1: 2) and PBS (pH 7.4) with the addition of 0.5% of a chitosan dispersion (80%), and evaluated the antifungal activity of S. nitens Bong. (Ruhland) derivatives against planktonic cells and biofilms of Candida parapsilosis. By a biomonitoring fractionation, the crude extract (EXT) and one fraction (F2) were selected and incorporated into a lipid nanoemulsion (NL) composed of cholesterol (10%), a 1:2 mixture of soybean phosphatidylcholine:polyoxyethylene-20-cetyl ether (10%), and phosphate buffer solution (pH 7.4) with a 0.5% chitosan dispersion (80%). The NL presented a diameter size between 50-200 nm, pseudoplastic behavior, and positive charge. The EXT and five fractions were active against planktonic cells. Results and Discussion: The incorporation of EXT and F2 into the NL increased antifungal activity and enhanced the anti-biofilm potential. This study classified the use of an NL as an important tool for the administration of S. nitens derivatives in cases of infections caused by this C. parapsisilosis. Conclusion: This work concluded that S. nitens derivatives were important sources of active molecules against C. parapsilosis and the use of a lipid nanoemulsion was an important tool to promote more effective F2 release and to improve the antifungal activity aiming the control of C. parapsilosis infections.
Department of Biological Sciences São Paulo State University (UNESP) School of Pharmaceutical Sciences, Campus Araraquara
Department of Drug and Medicines São Paulo State University (UNESP) School of Pharmaceutical Sciences, Campus Araraquara
Department of Organic Chemistry São Paulo State University (UNESP) Chemistry Institute, Campus Araraquara
Department of Biological Sciences São Paulo State University (UNESP) School of Pharmaceutical Sciences, Campus Araraquara
Department of Drug and Medicines São Paulo State University (UNESP) School of Pharmaceutical Sciences, Campus Araraquara
Department of Organic Chemistry São Paulo State University (UNESP) Chemistry Institute, Campus Araraquara
CAPES: 001
