Author(s): Rossoni, Rodnei Dennis [UNESP] ; de Barros, Patrícia Pimentel [UNESP] ; Lopes, Lucas Alexandre das Chagas [UNESP] ; Ribeiro, Felipe Camargo [UNESP] ; Nakatsuka, Toshiyuki ; Kasaba, Hideto ; Junqueira, Juliana Campos [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/201343
Origin: Oasisbr
Subject(s): biofilms; Candida spp; Galleria mellonella; S-PRG filler; biofilms; biofilms; Candida spp; Candida spp; Galleria mellonella; Galleria mellonella; S-PRG filler; S-PRG filler
Made available in DSpace on 2020-12-12T02:30:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-10-21
Surface pre-reacted glass-ionomer (S-PRG) is a bioactive filler produced by PRG technology, which is applied to various dental materials. The inhibitory effects of S-PRG eluate against Candida, the most common fungal oral pathogen, were investigated. Minimum inhibitory concentrations (MIC) and anti-biofilm activities were tested against Candida albicans, Candida glabrata, Candida krusei, and Candida tropicalis. For the in vivo study, Galleria mellonella was used as a model to evaluate the effects of S-PRG on toxicity, hemocyte counts and candidiasis. The MIC of S-PRG ranged from 5 to 40% (v/v). S-PRG eluate exhibited anti-biofilm activity for all the Candida species tested. Furthermore, injection of S-PRG eluate into G. mellonella was not toxic to the larvae and protected G. mellonella against experimental candidiasis. In addition, S-PRG eluate inhibited biofilm formation by C. albicans, C. glabrata, C. krusei, and C. tropicalis and exerted protective effects on G. mellonella against experimental candidiasis in vivo.
Department of Biosciences and Oral Diagnosis Institute of Science and Technology UNESP - Univ Estadual Paulista
Department of Research SHOFU Inc.
Department of Biosciences and Oral Diagnosis Institute of Science and Technology UNESP - Univ Estadual Paulista
