Detalhes do Documento

Use of the paired samples (cerebrospinal fluid and serum) in immunodiagnostic of active and inactive human neurocysticercosis

Autor(es): Barcelos, Ivanildes Solange da Costa ; Ferreira, Marcelo Simão ; Moura, Leandro Pajuaba de ; Biondi, Germano Francisco [UNESP] ; Costa-Cruz, Julia Maria

Data: 2014

Identificador Persistente: http://hdl.handle.net/11449/68299

Origem: Oasisbr

Assunto(s): Cerebrospinal fluid; Enzyme-linked immunosorbent assay; Neurocysticercosis; Serum; Taenia crassiceps; diagnostic agent; helminth antibody; immunoglobulin G; parasite antigen; animal; blood; case control study; cerebrospinal fluid; comparative study; cross reaction; enzyme linked immunosorbent assay; female; human; immunology; male; neurocysticercosis; Taenia solium; Animals; Antibodies, Helminth; Antigens, Helminth; Case-Control Studies; Cross Reactions; Enzyme-Linked Immunosorbent Assay; Female; Humans; Immunoglobulin G; Male; Cerebrospinal fluid; Cerebrospinal fluid; Enzyme-linked immunosorbent assay; Enzyme-linked immunosorbent assay; Neurocysticercosis; Neurocysticercosis; Serum; Serum; Taenia crassiceps; Taenia crassiceps; diagnostic agent; diagnostic agent; helminth antibody; helminth antibody; immunoglobulin G; immunoglobulin G; parasite antigen; parasite antigen; animal; animal; blood; blood; case control study; case control study; cerebrospinal fluid; cerebrospinal fluid; comparative study; comparative study; cross reaction; cross reaction; enzyme linked immunosorbent assay; enzyme linked immunosorbent assay; female; female; human; human; immunology; immunology; male; male; neurocysticercosis; neurocysticercosis; Taenia solium; Taenia solium; Animals; Animals; Antibodies, Helminth; Antibodies, Helminth; Antigens, Helminth; Antigens, Helminth; Case-Control Studies; Case-Control Studies; Cross Reactions; Cross Reactions; Enzyme-Linked Immunosorbent Assay; Enzyme-Linked Immunosorbent Assay; Female; Female; Humans; Humans; Immunoglobulin G; Immunoglobulin G; Male; Male


Descrição

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Paired samples of cerebrospinal fluid (CSF) and serum of 30 patients - 10 with active, 10 with inactive neurocysticercosis (NCC), and 10 control subjects - were evaluated by enzyme-linked immunosorbent assay (ELISA) using two Taenia crassiceps metacestode extracts as antigen in order to detect IgG antibodies. In active NCC, high levels of IgG were detected (p < 0.05). The CSF samples showed 80% (CI 72-88) of reactivity in the saline extract (S) and 90% (CI 84-95) in sodium dodecyl sulphate (SDS) and the serum samples were reactive in 90% (CI 84-95) and 100% (CI 98-100) in the S and SDS antigenic extracts, respectively. The use of the paired samples of CSF and serum in active NCC showed equivalent results suggesting that the serum samples could be used as a screening in those patients whose CSF puncture is counter-indicated.

Laboratório de Parasitologia Instituto de Ciências Biomédicas, 38400-902 Uberlândia, MG

Departamento de Clínica Médica Faculdade de Medicina Universidade Federal de Uberlândia, Av. Pará 1720, 38400-902 Uberlândia, MG

Departamento de Veterinária Higiene e Saúde Pública Universidade do Estado de São Paulo, Botucatu, SP

Tipo de Documento Artigo científico
Idioma Inglês
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