In vitro antimicrobial activity of extracts and essential oils of Cinnamomum, Salvia, and Menthaspp. against foodborne pathogens: a meta‐analysis study

• Author(s): Ezzaky, Youssef ; Elmoslih, Abdelkhaleq ; Silva, Beatriz Nunes ; Bonilla‐Luque, Olga M. ; Possas, Arícia ; Valero, Antonio ; Cadavez, Vasco ; Gonzales-Barron, Ursula ; Achemchem, Fouad
• Date: 2023
• Persistent ID: http://hdl.handle.net/10198/29342
• Origin: Biblioteca Digital do IPB
• Subject(s): Honey bee; Spermatheca; Genomic DNA isolation; DNA extraction
• Document Type: Journal article
• Language: English
• Contributor(s): Biblioteca Digital do IPB

Description

Genetic analysis of the honey bee spermathecal content can be particularly useful to provide an estimate of the genetic diversity and purity of the surrounding populations. Here we compared the concentration and quality of DNA extracted from queen spermatheca using four commercial kits to determine the best method to obtain DNA suitable for single nucleotide polymorphism genotyping by next-generation sequencing. The four kits were tested with different adjustments in the lysis incubation time, use of RNA-carrier, elution conditions and number of re-elutions. Only the use of QIAamp DNA Microkit with 3 h of lysis incubation, the addition of RNA-carrier and multiple re-elutions produced a DNA concentration over the required threshold.