Author(s): Alexandre, Nuno ; Gärtner, Andrea ; Amorim, Irina ; Santos, José D. ; Lopes, Maria Ascensão ; Colette Maurício, Ana ; Luís, Ana Lúcia
Date: 2010
Persistent ID: http://hdl.handle.net/10174/2334
Origin: Repositório Científico da Universidade de Évora
Subject(s): Vascular; grafts; sheep; polyvinyl alcohol hydrogel; artificial
Researchers developed this project according with the tasks originally planned, so far focusing their research activities in the biocompatibility and functionality of vascular implants. The biocompatibility was studied by following the ISO standard 10993-6 for evaluation of medical devices through the evaluation and grading of inflammation caused by implants placed subcutaneously. The implants were placed in sheep and collected randomly at different time points of the experimental period and subsequently processed histologically and classified according to Annex E to the standard mentioned above. The functionality of vascular implants has been evaluated to date by replacement of segments of the carotid by ePTFE implants with 5 cm long and 4 mm in diameter, coated or not with cultured stem cells. The deployment of these prostheses has been performed in carotid arteries of sheep and their functional performance measured by vascular ultrasound in Doppler and B mode by measuring parameters such as: peak systolic / diastolic blood flow velocity, vascular diameters at implantation and at the periphery of these. These measurements are performed at various time points of the experiment were folloed by euthanasia of sheep and immediately sample collection for the implementation of techniques like immunohistochemistry, morphometry and scanning electronic microscopy. Complications were observed throughout the experimental period include the formation of thrombi after at the transition implantcarotid region and vascular dilatation as the most frequent, respectively, in periods of short-and long-term experiment.
