Autor(es):
Singer, Wibke ; Manthey, Marie ; Panford-Walsh, Rama ; Matt, Lucas ; Geisler, Hyun-Soon ; Passeri, Eleonora ; Baj, Gabriele ; Tongiorgi, Enrico ; Leal, Graciano ; Duarte, Carlos B. ; Salazar, Ivan L. ; Eckert, Philipp ; Rohbock, Karin ; Hu, Jing ; Strotmann, Jörg ; Ruth, Peter ; Zimmermann, Ulrike ; Rüttiger, Lukas ; Ott, Thomas ; Schimmang, Thomas ; Knipper, Marlies
Data: 2018
Identificador Persistente: https://hdl.handle.net/10316/107624
Origem: Estudo Geral - Universidade de Coimbra
Projeto/bolsa:
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID/PT;
Assunto(s): BDNF detection; Bdnf exon-IV and -VI; activity-dependent BDNF expression; long-lasting plasticity changes; transgenic BDNF reporter mice
Descrição
Bdnf exon-IV and exon-VI transcripts are driven by neuronal activity and are involved in pathologies related to sleep, fear or memory disorders. However, how their differential transcription translates activity changes into long-lasting network changes is elusive. Aiming to trace specifically the network controlled by exon-IV and -VI derived BDNF during activity-dependent plasticity changes, we generated a transgenic reporter mouse for B DNF- l ive- e xon- v isualization (BLEV), in which expression of Bdnf exon-IV and -VI can be visualized by co-expression of CFP and YFP. CFP and YFP expression was differentially activated and targeted in cell lines, primary cultures and BLEV reporter mice without interfering with BDNF protein synthesis. CFP and YFP expression, moreover, overlapped with BDNF protein expression in defined hippocampal neuronal, glial and vascular locations in vivo. So far, activity-dependent BDNF cannot be explicitly monitored independent of basal BDNF levels. The BLEV reporter mouse therefore provides a new model, which can be used to test whether stimulus-induced activity-dependent changes in BDNF expression are instrumental for long-lasting plasticity modifications.
This work was supported by the Deutsche Forschungs gemeinschaft DFG-Kni-316-10-1 (RP-W; WS; H-SG; EP; MK; TO); FOR 2060 project RU 713/3-2 (LR); SPP 1608 RU 316/12-1; KR; H-SG); KN 316/12-1 (MM; UZ; MK); the Brain and Behavior Research Foundation NARSAD Young Investigator Grant 20748 (LM), BFU2013-40944 (TS); DFG-STR 619/5-1 (JS); CIN-EXC 307 (JH); DFG KFO134 (PR); PRIN2010-11 2010N8PBAA (GB; ET); Pest-C/SAU/LA0001/2013-2014, CENTRO-01-0145-FEDER-000008 (CENTRO2020), POCI01-0145-FEDER-007440 and POCI-01-0145-FEDER-028656 (COMPETE2020 and FCT), UID/NEU/04539/2013, and UID/BIM/4501/2013 (GL; ILS; CBD).
