Autor(es):
Baptista, Letícia de Carvalho, 1990- ; Figueira, Camilla Olivares, 1982- ; Souza, Bruno Batista, 1983- ; Tavares, Arthur Antolini ; Costa, Fernando Ferreira, 1950- ; Melo, Mônica Barbosa de, 1968- ; Nascimento, Maria Laura Costa do, 1979-
Data: 2019
Identificador Persistente: https://hdl.handle.net/20.500.12733/1643947
Origem: Oasisbr
Assunto(s): Gravidez; Pré-eclâmpsia; Anemia falciforme; Placenta; Placenta; Sickle cell anemia; Pre-Eclampsia; Pregnancy; Gene expression; Artigo original
Descrição
Agradecimentos: This study was supported by São Paulo Research Foundation (FAPESP) grant 2015/08330-5, grant 2014/00984-3 and the National Council for Scientific and Technological Development (CNPq) grant 409605/2016-6
Abstract: Environmentally induced changes in placental morphological and molecular phenotypes may provide relevant insight towards pathophysiology of diseases. Sickle cell disease (SCD) is a common inherited hemoglobin disorder characterized by chronic hemolytic anemia and vaso-occlusive crisis. SCD leads to higher morbidity and mortality, especially during pregnancy, with increased risk of preeclampsia (PE). To compare clinical findings, placental morphology, and gene expression in villous placental tissue using next generation sequencing. We included five cases. Two placentas from the same woman with homozygous SCD that had been pregnant twice and had different maternal and fetal outcomes (one early onset PE/eclampsia and a mostly non-complicated pregnancy); an early onset PE, a fetal growth restriction and a term, non-complicated pregnancy. Sixty-four differentially expressed genes were observed in the SCD+PE case, in comparison with the placenta from the SCD without PE, based on fold change. Among these genes, 59 were upregulated and 5 were downregulated. Enrichment analysis indicated two significant biological processes: response to copper ion (CYP1A1, AOC1, AQP1, and ATP5D) and triglyceride-rich lipoprotein particle clearance (GPIHBP1, APOC1, and APOE). The rare opportunity to evaluate the same patient in two different pregnancies, of opposing outcomes, and compare to other conditions of known placental and vascular/inflammatory involvement, may further the understanding of the pathophysiology of PE in SCD. Our results suggest that the clinical association between SCD and PE may be supported by common pathophysiological mechanisms, but that pathways involving response to copper and triglyceride metabolism could be important drivers of PE pathophysiology
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
Fechado
