Author(s): Dutra, Alexandre Augusto de Assis ; Sousa, Lucas Oliveira ; Resende, Rodrigo Ribeiro ; Brandão, Rogélio Lopes ; Kalapothakis, Evanguedes ; Castro, Ieso de Miranda
Date: 2012
Origin: Oasisbr
Subject(s): Calcium channel; Venom; Spider toxins; Lasiodora; cDNA; Expression
Here, we described the expression and characterization of the recombinant toxin LTx2, which was previously isolated from the venomous cDNA library of a Brazilian spider, Lasiodora sp. (Mygalomorphae, Theraphosidae). The recombinant toxin found in the soluble and insoluble fractions was purified by reverse phase high-performance liquid chromatography (HPLC). Ca2+ imaging analysis revealed that the recombinant LTx2 acts on calcium channels of BC3H1 cells, blocking L-type calcium channels.
