Author(s): Praça, Milene Miranda ; Carvalho, Carlos Roberto de ; Clarindo, Wellington Ronildo
Date: 2018
Origin: Oasisbr
Subject(s): Colchicine; Chromosome; Flow cytometry; Solanum lycopersicum
Document details
A practical and reliable procedure for in vitro induction of tetraploid tomato
Description
A relatively rapid, practical and reliable procedure for in vitro production of tetraploid tomato using colchicine is described. Using this procedure 11.11% generation of tetraploids was obtained after exposure of seedling shoot meristem explants with 8 mM colchicine for 96 h. Confirmation of tetraploid production (2n = 48), was determined by flow cytometry and verified by cytogenetic analysis of root tip preparations. The results indicate that the procedure is adequate for induction and screening of tetraploid tomato plantlets.
Document Type
Journal article
Language English
Language English
