Autor(es):
Feio, Danielle Cristina Azevedo ; Oliveira, Nayara Cristina Lima de ; Pereira, Edmundo Luis Rodrigues ; Morikawa, Aleksandra Tiemi ; Muniz, Jos? Augusto Pereira Carneiro ; Montenegro, Raquel Carvalho ; Alves, Ana Paula Negreiros Nunes ; Lima, Patricia Danielle Lima de ; Maranh?o, Raul Cavalcante ; Burbano, Rommel Rodr?guez
Data: 2017
Origem: Oasisbr
Assunto(s): Cebus / anatomia & histologia; Cebus / sangue; Paclitaxel / administra??o & dosagem; Paclitaxel / efeitos adversos; Paclitaxel / farmacocin?tica; Paclitaxel / toxicidade; Paclitaxel / uso terap?utico; Emuls?es Gordurosas Intravenosas / administra??o & dosagem; Emuls?es Gordurosas Intravenosas / efeitos adversos; Emuls?es Gordurosas Intravenosas / toxicidade; Emuls?es Gordurosas Intravenosas / farmacocin?tica; Emuls?es Gordurosas Intravenosas / uso terap?utico; Quimioterapia Combinada / efeitos adversos; Avalia??o Pr?-Cl?nica de Medicamentos / veterin?ria; An?lise Qu?mica do Sangue / veterin?ria; Efeitos Colaterais e Rea??es Adversas Relacionados a Medicamentos; An?lise de Vari?ncia; Nanopart?culas; Lip?deos
Descrição
Federal University of Par?. Institute of Biological Sciences. Human Cytogenetics Laboratory. Bel?m, PA, Brazil.
Federal University of Par?. Institute of Biological Sciences. Human Cytogenetics Laboratory. Bel?m, PA, Brazil.
Federal University of Par?. Institute of Biological Sciences. Human Cytogenetics Laboratory. Bel?m, PA, Brazil.
University of S?o Paulo. Heart Institute. Medical School Hospital. S?o Paulo, SP, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil.
Federal University of Par?. Institute of Biological Sciences. Human Cytogenetics Laboratory. Bel?m, PA, Brazil.
Federal University of Cear?. Health Sciences Center. Department of Clinical Dentistry. Fortaleza, CE, Brazil.
State University of Par?. Post Graduate Program of Amazon Parasitic Biology, Biological and Health Sciences Center. Molecular Biology Laboratory. Bel?m, PA, Brazil.
University of Sao Paulo. Heart Institute. Medical School Hospital . S?o Paulo, SP, Brazil.
Federal University of Par?. Institute of Biological Sciences. Human Cytogenetics Laboratory. Bel?m, PA, Brazil.
Lipid-based nanoparticle systems have been used as vehicles for chemotherapeutic agents in experimental cancer treatments. Those systems have generally been credited with attenuating the severe toxicity of chemotherapeutic agents. This study aimed to investigate the effects of associating paclitaxel (PTX) with a lipid-based nanoparticle system on a nonhuman primate, Cebus apella, documenting the toxicity as measured by serum biochemistry, which is a detailed analysis of blood and tissue. Eighteen C. apella were studied: three animals were treated with cholesterol-rich nanoemulsion (LDE) only, without PTX, administered intravenously every 3 weeks, during six treatment cycles; six animals were treated with PTX associated with LDE at the same administration scheme, three with lower (175 mg/m2 ) and three with higher (250 mg/m2 ) PTX doses; and six animals were treated with commercial PTX, three with the lower and three with the higher doses. In the LDE-PTX group, no clinical toxicity appeared, and the weight?food consumption curve was similar to that of the controls. Two animals treated with commercial PTX presented weight loss, nausea and vomiting, diarrhea, skin flaking, 70% loss of body hair, and decreased physical activity. The use of LDE as a carrier at both lower and higher doses reduced the toxicity of the drug in this species, which is closely related to human subjects. This was observed not only by clinical, biochemical, and hematological profiles but also by the histopathological analysis. The results of this study support the assumption that lipid-based nanoparticle systems used as drug carriers can serve as valuable tools to decrease the toxicity and increase the safety of chemotherapeutic agents.
