Author(s): Lima, Luana Nepomuceno Costa ; Frota, Cristiane Cunha ; Suffys, Philip Noel ; Fontes, Amanda Nogueira Brum ; Mota, Rosa Maria Salani ; Almeida, Rosa Livia Freitas ; Pontes, Maria Araci de Andrade ; Gon?alves, Heitor de S? ; Kendall, Carl ; Kerr, Ligia Regina Sansigolo
Date: 2018
Origin: Oasisbr
Subject(s): Hansen?ase / diagn?stico; Mycobacterium leprae / patogenicidade; Cavidade Nasal / patologia; Rea??o em Cadeia da Polimerase / m?todos; Gen?tipo; Biomarcadores
Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico, Brazil under [grant number CNPq?410573/2006-0].
Federal University of Cear?. Faculdade de Medicina. Departamento de Patologia e Medicina Legal. Fortaleza, CE, Brazil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Federal University of Cear?. Faculdade de Medicina. Departamento de Patologia e Medicina Legal. Fortaleza, CE, Brazil.
Funda??o Osvaldo Cruz. Instituto Oswaldo Cruz. Laborat?rio de Biologia Molecular Aplicada a Micobact?rias. Rio de Janeiro, RJ, Brasil.
Funda??o Osvaldo Cruz. Instituto Oswaldo Cruz. Laborat?rio de Biologia Molecular Aplicada a Micobact?rias. Rio de Janeiro, RJ, Brasil.
Federal University of Cear?. Departamento de Estat?stica e Matem?tica Aplicada. Fortaleza, CE, Brazil.
Universidade de Fortaleza. Programa de P?s-Gradua??o em Sa?de Coletiva. Fortaleza, CE, Brazil.
Centro de Dermatologia Dona Lib?nia. Department of the State of Cear?. Fortaleza, CE, Brazil.
Centro de Dermatologia Dona Lib?nia. Department of the State of Cear?. Fortaleza, CE, Brazil.
Tulane University. School of Public Health and Tropical Medicine. Department of Global Community Health and Behavioral Sciences. New Orleans , LA , USA.
Federal University of Cear?. Faculdade de Medicina. Departamento de Sa?de Comunit?ria. Fortaleza, CE, Brazil.
This study analyzed the genetic diversity by MIRU-VNTR of Mycobacterium leprae isolates from nasal cavities and related to epidemiological and clinical data. The sample consisted of 48 newly diagnosed leprosy cases that tested positive for M. leprae PCR in nasal secretion (NS) attending to the National Reference Center of Dermatology Dona Libania (CDERM), Fortaleza, Brazil. Total DNA was extracted from NS of each patient and used for amplification of four M. leprae VNTR loci. Four clusters of M. leprae isolates were formed with identical genotypes. In the spatial analysis, 12 leprosy cases presented similar genotypes organized into 4 clusters. The most common genotypes in the current study was AC8b: 8, AC9: 7, AC8a: 8, GTA9: 10, which may represent a genotype of circulating strains most often in Cear?. A minimum set of four MIRU-VNTR loci was demonstrated to study the genetic diversity of M. leprae isolates from NS.
