Autor(es):
Oliveira, Aline Nascimento ; Pinheiro, Alana Miranda ; Bel?m-Filho, Ivaldo Jesus Almeida ; Fernandes, Luanna Melo Pereira ; Cart?genes, Sabrina Carvalho ; Ribera, Paula Cardoso ; Fontes-J?nior, En?as Andrade ; Crespo-Lopez, Maria Elena ; Monteiro, Marta Chagas ; Lima, Marcelo Oliveira ; Maia, Cristiane Socorro Ferraz
Data: 2018
Origem: Oasisbr
Assunto(s): Compostos de Metilmerc?rio / toxicidade; Merc?rio / envenenamento; Sistema Nervoso Central / fisiopatologia; C?rtex Motor / efeitos dos f?rmacos; Etanol / envenenamento; Ratos / metabolismo
Descrição
This work was funded by Funda??o Amaz?nia de Amparo a Estudos e Pesquisa do Par? (FAPESPA, grant number 160/2014 Edital 006/2014). Bel?m-Filho IJA and Nascimento AS received scholarships from Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES)
Universidade Federal do Par?. Faculdade de Ci?ncias Farmac?uticas. Laborat?rio de Farmacologia da Inflama??o e do Comportamento. Bel?m, PA, Brazil.
Universidade Federal do Par?. Faculdade de Ci?ncias Farmac?uticas. Laborat?rio de Farmacologia da Inflama??o e do Comportamento. Bel?m, PA, Brazil.
Universidade Federal do Par?. Faculdade de Ci?ncias Farmac?uticas. Laborat?rio de Farmacologia da Inflama??o e do Comportamento. Bel?m, PA, Brazil.
Universidade Federal do Par?. Faculdade de Ci?ncias Farmac?uticas. Laborat?rio de Farmacologia da Inflama??o e do Comportamento. Bel?m, PA, Brazil.
Universidade Federal do Par?. Faculdade de Ci?ncias Farmac?uticas. Laborat?rio de Farmacologia da Inflama??o e do Comportamento. Bel?m, PA, Brazil.
Universidade Federal do Par?. Faculdade de Ci?ncias Farmac?uticas. Laborat?rio de Farmacologia da Inflama??o e do Comportamento. Bel?m, PA, Brazil.
Universidade Federal do Par?. Faculdade de Ci?ncias Farmac?uticas. Laborat?rio de Farmacologia da Inflama??o e do Comportamento. Bel?m, PA, Brazil.
Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Laborat?rio de Farmacologia Molecular. Bel?m, PA, Brazil.
Universidade Federal do Par?. Faculdade de Ci?ncias Farmac?uticas. Laborat?rio de Microbiologia e Imunologia Clinica. Bel?m, PA, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Toxicologia. Ananindeua, PA, Brasil.
Universidade Federal do Par?. Faculdade de Ci?ncias Farmac?uticas. Laborat?rio de Farmacologia da Inflama??o e do Comportamento. Bel?m, PA, Brazil.
Methylmercury (MeHg) is a hazardous environmental pollutant, affecting Amazon basin communities by anthropogenic activities. The exact safe level of MeHg exposure is unclear, despite the efforts of health international societies to avoid mercury (Hg) poisoning. Central nervous system is severely impacted by Hg intoxication, reflecting on motor impairment. In addition, alcohol has been associated to an overall brain damage. According to lifestyle of Amazon riverside communities, alcohol intake occurs frequently. Thus, we investigated if continuous MeHg exposure at low doses during adolescence displays motor deficits (experiment 1). In the experiment 2, we examine if the co-intoxication (i.e. MeHg plus ethanol exposure) during adolescence intensify motor damage. In the experiment 1, Wistar adolescent rats (31 days old) received chronic exposure to low dose (CELD) of MeHg (40 ?g/kg/day) for 35 days. For the experiment 2, five sessions of alcohol binge drinking paradigm (3ON-4OFF; 3.0 g/kg/day) were employed associated to MeHg intoxication. Motor behaviour was evaluated by the open field, pole test, beam walking and rotarod paradigms. CELDS of MeHg display motor function damage, related to hypoactivity, bradykinesia-like behaviour, coordination deficits and motor learning impairment. Co-intoxication of MeHg plus ethanol reduced cerebellar Hg content, however also resulted in motor behavioural impairment, as well as additive effects on bradykinesia and fine motor evaluation.
