Enhanced detection with prussian blue-based nanocubes: a novel electrochemical biosensor for bovine serum albumin analysis: microfluidic integration

• Author(s): Santos, Andréa dos ; Oliveira, Daniela ; Martins, Gabriela ; Moreira, Felismina T.C.
• Date: 2024
• Persistent ID: http://hdl.handle.net/10400.22/26371
• Origin: Repositório Científico do Instituto Politécnico do Porto
• Subject(s): Biomarkers; Survival rates; Electrochemical biosensor
• Document Type: Conference object
• Language: English
• Contributor(s): REPOSITÓRIO P.PORTO

Description

(Introduction) Early diagnosis of biomarkers is crucial for optimizing treatments and increasing survival rates. Direct and cost-effective assays are important for point-of-care (PoC) testing. Bovine serum albumin (BSA) monitoring indicates liver and kidney function and aids in the assessment of diseases such as cirrhosis and chronic kidney disease. An innovative electrochemical biosensor was developed in which a platinum electrode was decorated with manganese-based Prussian blue nanocubes (PB-NC). A molecularly imprinted polymer (MIP) was created on the electrode surface by electropolymerization of phenol in the presence of BSA using cyclic voltammetry (CV). The template was removed with proteinase K and a mixture of methanol and acetic acid. Square wave voltammetry (SWV) characterized the performance of the sensor, which exhibited high sensitivity and allowed detection of BSA at nanomolar levels.