TBCCD1 is a key regulator of centrosomal microtubule anchor and basal body positioning/attachment

• Author(s): Carmona, Bruno ; Camelo, Carolina ; Mehraz, Manon ; Lemullois, Michel ; Nolasco, Sofia ; Marinho, H. Susana ; Tassin, Anne-Marie ; Koll, France ; Soares, Helena
• Date: 2018
• Persistent ID: http://hdl.handle.net/10400.21/10029
• Origin: Repositório Científico do Instituto Politécnico de Lisboa
• Subject(s): Centrosomes; Basal body; Ciliogenesis
• Document Type: Conference object
• Language: English
• Contributor(s): RCIPL

Description

Successful cilia assembly requires correct positioning and anchoring of the centrosome's mother centriole/basal body (BB) to the cell membrane. A clear picture of the different signals and players involved in centrosome positioning/anchoring is still not available. Published work from our group identified a new TBCC domain-containing human protein (TBCCD1). Depletion of TBCCD1 in human RPE-1 cells severely affects the relative position of the centrosome to the nucleus and the efficiency of cells to assemble primary cilia. Aim of the study: To dissect the mechanisms involving TBCCD1 in human RPE-1 positioning and anchoring during ciliogenesis.