Autor(es):
Sebastião, Cruz S. ; Neto, Zoraima ; Jesus, Carlos S. de ; Mirandela, Marinela ; Jandondo, Domingos ; Couto-Fernandez, José C. ; Tanuri, Amilcar ; Morais, Joana ; Brito, Miguel
Data: 2019
Identificador Persistente: http://hdl.handle.net/10400.21/10757
Origem: Repositório Científico do Instituto Politécnico de Lisboa
Assunto(s): HIV infection; Pregnancy; Antiretroviral therapy; Antimicrobial resistance; Epidemiology; Human genetics; Angola; Luanda; Project Pró-África CNPq n˚ 440145/2015-5
Descrição
Monitoring genetic diversity and drug resistance mutations (DRMs) is critical for understanding HIV epidemiology. Here, we report HIV-1 genetic diversity and DRMs in blood samples from 42 HIV-positive pregnant women naive to antiretroviral therapy (ART), in Luanda. The samples were subjected to nested-PCR, followed by sequencing of the HIV-1 pol gene, targeting the protease and reverse transcriptase fragments. HIV-1 diversity was analyzed using the REGA HIV-1 subtyping tool and DRMs were identified using the Calibrated Population Resistance tool. A total of 34 sequences were obtained. The data revealed wide HIV-1 subtypes heterogeneity, with subtype C (38%, 13/34) the most frequent, followed by the subtypes F1 (18%, 6/34), A1 (9%, 3/34), G (9%, 3/34), D (6%, 2/34) and H (3%, 1/34). In addition, recombinants strains were detected, with CRF02_AG (6%, 2/34) the most frequent, followed by CRF37_cpx, F1/C, A1/G, and H/G, all with 3% (1/34). A total of 6/34 (18%) of the sequences presented DRMs. The non-nucleoside reverse transcriptase inhibitors presented 15% (5/34) of resistance. Moreover, 1/34 (3%) sequence presented resistance against both non-nucleoside reverse transcriptase inhibitors and nucleoside reverse transcriptase inhibitors, simultaneously. Despite the small sample size, our results suggest the need to update currently used ART regimens. Surveillance of HIV-1 subtypes and DRMs are necessary to understand HIV epidemiology and to guide modification of ART guidelines in Angola.
