Autor(es): Fontes, Ana Luiza ; Neves, Bruna ; Conde, Tiago ; Couto, Daniela ; Pimentel, Lígia Leão ; Rodríguez-Alcalá, Luis Miguel ; Domingues, M. Rosário ; Gomes, Ana Maria
Data: 2024
Identificador Persistente: http://hdl.handle.net/10400.14/42311
Origem: Veritati - Repositório Institucional da Universidade Católica Portuguesa
Assunto(s): Conjugated linoleic acid; Fatty acid metabolites; Bifidobacterium breve
Microbially enriched food in conjugated linoleic (CLA) and conjugated linolenic (CLNA) acids is intensively studied nowadays. The conversion of linoleic (LA) and α-linolenic acids (α-LNA) into these compounds may involve different fatty acid (FA) intermediates. This research aimed to investigate potential FA byproducts in milk during microbial CLA/CLNA-enrichment using Bifidobacterium breve DSM 20091. Milk fermented with pure α-LNA showed a decrease in free myristic acid, while pure LA led to an increase in free stearic acid. No additional FA compounds were found alongside CLA/CLNA isomers. The strain produced several CLA isomers from LA, but only when administered alone. Nonetheless, when α-LNA was assayed, additional CLNA isomers, never reported before for bifidobacteria, were observed. In conclusion, except for stearic acid in the presence of LA, no side-FA metabolites were released during milk microbial CLA/CLNA-enrichment. Results suggest either CLA/CLNA production occurs in one single-step or intermediates biotransformation is very fast.
