Potential of KM+ lectin in immunization against Leishmania amazonensis infection

• Author(s): Teixeira, Clarissa Romero ; Teixeira, Maria Jania ; Barral-Netto, Manoel ; Barral, Aldina Maria Prado ; Cavassani, Karen A. ; Gomes, Regis Bernardo Brandim ; Barreira, Maria Cristina Roque ; Cavada, Benildo Sousa ; Silva, João Santana da ; Teixeira, Clarissa Romero ; Teixeira, Maria Jania ; Barral-Netto, Manoel ; Barral, Aldina Maria Prado ; Cavassani, Karen A. ; Gomes, Regis Bernardo Brandim ; Barreira, Maria Cristina Roque ; Cavada, Benildo Sousa ; Silva, João Santana da
• Date: 2013
• Origin: Oasisbr
• Subject(s): Leishmania; Lectin; Bone-marrow-derived dendritic cells (BMDC)
• Document Type: Journal article
• Language: English

Description

Texto completo: acesso restrito. p. 3001–3008

Submitted by Edileide Reis ([e-mail]) on 2013-08-27T12:32:25Z No. of bitstreams: 1 1-s2.0-S0264410X05012892-main.pdf: 364811 bytes, checksum: a7aa8706c19f35fd548a6e19bdd08a62 (MD5)

Approved for entry into archive by Rodrigo Meirelles ([e-mail]) on 2013-11-19T20:37:56Z (GMT) No. of bitstreams: 1 1-s2.0-S0264410X05012892-main.pdf: 364811 bytes, checksum: a7aa8706c19f35fd548a6e19bdd08a62 (MD5)

Made available in DSpace on 2013-11-19T20:37:56Z (GMT). No. of bitstreams: 1 1-s2.0-S0264410X05012892-main.pdf: 364811 bytes, checksum: a7aa8706c19f35fd548a6e19bdd08a62 (MD5) Previous issue date: 2006

In the present study we evaluated Canavalia brasiliensis (ConBr), Pisum arvense (PAA) and Artocarpus integrifolia (KM+) lectins as immunostimulatory molecules in vaccination against Leishmania amazonensis infection. Although they induced IFN-γ production, the combination of the lectins with SLA antigen did not lead to lesion reduction. However, parasite load was largely reduced in mice immunized with KM+ lectin and SLA. KM+ induced a smaller inflammatory reaction in the air pouch model and was able to inhibit differentiation of dendritic cells (BMDC), but to induce maturation by enhancing the expression of MHC II, CD80 and CD86. These observations indicate the modulatory role of plant lectins in leishmaniasis vaccination may be related to their action on the initial innate response.