Author(s): Zolotareva, Maria ; Cascalheira, Francisco ; Caneiras, Catia ; Bárbara, Cristina ; Caetano, Diogo Miguel ; Teixeira, Miguel Cacho
Date: 2024
Persistent ID: http://hdl.handle.net/10400.5/97392
Origin: Repositório da Universidade de Lisboa
Subject(s): Biosensors; Fungal infection; Microfluidics; Molecular diagnosis
The diagnosis of fungal infections presents several challenges and limitations, stemming from the similarities in symptomatology, diversity of underlying pathogenic species, complexity of fungal biology, and scarcity of rapid, affordable, and point-of-care approaches. In this review, we assess technological advances enabling the conversion of cutting-edge laboratory molecular diagnostic methods to cost-effective microfluidic devices. The most promising strategies toward the design of DNA sequence-based fungal diagnostic systems, capable of capturing and deciphering the highly informative DNA of the pathogen and adapted for resource-limited settings, are discussed, bridging fungal biology, molecular genetics, microfluidics, and biosensors.
