Autor(es): Herdade, Ana Santos Silva ; Andolina, Giulia ; Faggio, Caterina ; Calado, Ângelo ; Saldanha, Carlota
Data: 2016
Identificador Persistente: http://hdl.handle.net/10451/34186
Origem: Repositório da Universidade de Lisboa
Assunto(s): Inflammation; Erythrocyte deformability; Nitric oxide; Intravital microscopy
We aim to establish an in vivo animal model of acute inflammation using PAF (platelet activating factor) as inflammatory agent and to study the erythrocyte deformability changes induced by the inflammatory response. Counting the number of rolling and adherent neutrophils to endothelium after 2, 4 and 6 h of intrascrotal injection of PAF we showed the induction of an inflammatory state. Blood samples are collected in order to measure the erythrocyte deformability and to quantify NO efflux from the red blood cells (RBCs). The results show an increased number of rolling and adherent neutrophils after 2 h and 4 h of inflammation aswell as decreased values of erythrocyte deformability in the same time-points. This result is in linewith the need of a lowblood viscosity to the recruitment process thatwill improve leukocyte migration towards the endothelial wall. NO efflux fromRBCs is also affected by the inflammatory response at the first hours of inflammation. This animal model demonstrates in vivo the association between an acute inflammatory response and the rheological properties of the blood, namely the RBCs deformability. For those reasons we consider this as an adequate model to study acute inflammatory responses as well as hemorheological parameters.
