Descrição
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Programa de Doutorado Sanduiche no Exterior (PDSE)
Programa de Apoio ao Desenvolvimento Cientifico da Faculdade de Ciencias Farmaceuticas da UNESP (PADC/FCF)
Pro-Reitora de Pesquisa da UNESP (PROPe/UNESP)
The available antifungal therapeutic arsenal is limited. The search for alternative drugs with fewer side effects and new targets remains a major challenge. Decyl gallate (G14) is a derivative of gallic acid with a range of biological activities and broad-spectrum antifungal activity. Previously, our group demonstrated the promising anti-Paracoccidioides activity of G14. In this work, to evaluate the antifungal characteristics of G14 for Paracoccidioides lutzii, a chemical-genetic interaction analysis was conducted on a Saccharomyces cerevisiae model. N-glycosylation and/or the unfolded protein response pathway was identified as a high-confidence process for drug target prediction. The overactivation of unfolded protein response (UPR) signaling was confirmed using this model with IRE1/ATF6/PERK genes tagged with green fluorescent protein (GFP). In P. lutzii, this prediction was confirmed by the low activity of glycosylated enzymes [alpha-(1,3)-glucanase, N-acetyl-alpha-D-glucosaminidase (NAGase), and alpha-(1,4)-amylase], by hyperexpression of genes involved with the UPR and glycosylated enzymes, and by the reduction in the amounts of glycosylated proteins and chitin. All of these components are involved in fungal cell wall integrity and are dependent on the N-glycosylation process. This loss of integrity was confirmed by the reduction in mitochondrial activity, impaired budding, enhancement of wall permeability, and a decrease in viability. These events led to a reduction of the ability of fungi to adhere on human lung epithelial cells (A549) in vitro. Therefore, G14 may have an important role in balancing the inflammatory reaction caused by fungal infection, without interfering with the microbicidal activity of nitric oxide. This work provides new information on the activity of G14, a potential anti-Paracoccidioides compound.
Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Clin Anal, Araraquara, SP, Brazil
Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Biol Sci, Araraquara, SP, Brazil
Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biochem & Immunol, Ribeirao Preto, SP, Brazil
Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Cellular Mol Biol & Pathogen Bioagents, Ribeirao Preto, SP, Brazil
Univ Toronto, Terrence Donnelly Ctr Cellular & Biomol Res, Dept Mol Genet, Toronto, ON, Canada
RIKEN Ctr Sustainable Resource Sci, Saitama, Japan
Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Clin Anal, Araraquara, SP, Brazil
Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Biol Sci, Araraquara, SP, Brazil
CNPq: 403586/2012-7
FAPESP: 2016/17048-4
FAPESP: 2015/03700-9
FAPESP: 2015/14023-8
FAPESP: 2013/10917-9
