Author(s):
Dos Santos, Amanda R. [UNESP] ; Fraga-Silva, Thais F. [UNESP] ; Almeida, Débora de F [UNESP] ; Dos Santos, Rodolfo F. [UNESP] ; Finato, Angela C. [UNESP] ; Amorim, Bárbara C [UNESP] ; Andrade, Maria I. ; Soares, Cleverson T. ; Lara, Vanessa S. ; Almeida, Nara Lm ; de Arruda, Olavo S. [UNESP] ; de Arruda, Maria Sp [UNESP] ; Venturini, James [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/199246
Origin: Oasisbr
Subject(s): BALB/c; C57BL/6; cytokines; IL-17; Il17ra-/-; il6; immunocompetent model; intravenous infection; mucormycosis; Rhizopus oryzae; rorc; Swiss
Description
Made available in DSpace on 2020-12-12T01:34:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-06-01
Aim: To investigate the immune response of disseminated Ryzopus oryzae infection in immunocompetent mice. Methods: C57Bl/6, BALB/c and Swiss wild-type mice were intravenously infected with R. oryzae; the parameters of infection and immune response were determined. Transcriptional signature of Th17 immune response and infection in Il17ra-/- mice were also evaluated. Results: All mouse strains showed an initial spread of R. oryzae in the target tissues; however, after 30 days, C57Bl/6 and BALB/c mice showed an effective fungal clearance associated with specific production of IL-17 and IL-2. We also observed that 60% of Il17ra-/- mice succumbed to infection within 16 days. Conclusion: This study has established an immunocompetent model for disseminated mucormycosis and highlighted the role of IL-17 signaling in immunity against R. oryzae.
Universidade Estadual Paulista (Unesp) Faculdade de Ciências
Universidade Estadual Paulista (Unesp) Instituto de Biociências
Lauro de Souza Lima Institute
Universidade de São Paulo (USP), Faculdade de Odontologia de Bauru (FOB)
Universidade Federal de Mato Grosso do Sul (UFMS) Faculdade de Medicina
Universidade Estadual Paulista (Unesp) Faculdade de Ciências
Universidade Estadual Paulista (Unesp) Instituto de Biociências
